Jin Baofang, Sun Dalin, Dong Weihang, Chen Bing, Deng Weimin, Cai Bin, Cui Yugui, Jin Yihan, Liu Jianguo, Tong Li, Wu Ping
Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
Medical College, Qinghai University, Xi'ning 810001, China.
Evid Based Complement Alternat Med. 2020 Apr 25;2020:1957267. doi: 10.1155/2020/1957267. eCollection 2020.
The testicular microcirculation was an important aspect of testicular physiology and it offered a stable environment for the transport of nutrients and secretary products in the testis. Yangjing capsule (YC), a traditional Chinese compound herbal prescription, has been proved as an effective drug to ameliorate spermatogenesis, promote testosterone synthesis in vivo, and cure spermatogenesis in clinical practice.
This study was aimed at understanding the potential mechanisms of YC exerting angiogenic effects in the mouse spermatogenesis dysfunction model induced by cyclophosphamide (CP) and MLTC-1 cells.
Balb/c mice were randomly divided into five groups: control, CP, CP plus YC (630 mg/kg), CP plus YC (1260 mg/kg), and CP plus YC (2520 mg/kg). After 30 days, mice were sacrificed and the expressions of endothelial marker CD34+, angiogenic marker VEGFA, VEGFR1, VEGFR2, and eNOS in the testes of the mice were examined; moreover, Leydig cell line MLTC-1 cells were cultured and treated with different concentrations of YC extracts (YCE), and the expressions of VEGFA, VEGFR1, VEGFR2, and eNOS, as well as the secretion of NO, were evaluated.
We observed that YC significantly increased the expressions of VEGFA, VEGFR1, VEGFR2, and eNOS in testes of CP-treated mice; moreover, YCE has led to increased expressions of VEGFA, VEGFR1, VEGFR2, and eNOS and secretion of NO in MLTC-1 . These data suggested that the YC might be an alternative treatment for the dysfunction of testicular microcirculation by promoting the angiogenesis in the testis.
睾丸微循环是睾丸生理学的一个重要方面，它为睾丸内营养物质和分泌产物的运输提供了稳定的环境。养精胶囊（YC）是一种传统的复方中药制剂，在临床实践中已被证明是一种改善精子发生、促进体内睾酮合成以及治疗精子发生的有效药物。
本研究旨在了解养精胶囊在环磷酰胺（CP）和MLTC-1细胞诱导的小鼠精子发生功能障碍模型中发挥血管生成作用的潜在机制。
将Balb/c小鼠随机分为五组：对照组、CP组、CP加YC（630mg/kg）组、CP加YC（1260mg/kg）组和CP加YC（2520mg/kg）组。30天后，处死小鼠，检测小鼠睾丸中内皮标志物CD34+、血管生成标志物VEGFA、VEGFR1、VEGFR2和eNOS的表达；此外，培养Leydig细胞系MLTC-1细胞，并用不同浓度的养精胶囊提取物（YCE）处理，评估VEGFA、VEGFR1、VEGFR2和eNOS的表达以及NO的分泌。
我们观察到，养精胶囊显著增加了CP处理小鼠睾丸中VEGFA、VEGFR1、VEGFR2和eNOS的表达；此外，养精胶囊提取物导致MLTC-1中VEGFA、VEGFR1、VEGFR2和eNOS的表达增加以及NO的分泌增加。这些数据表明，养精胶囊可能通过促进睾丸血管生成，成为治疗睾丸微循环功能障碍的一种替代疗法。
