Goren M R, Kilinc F, Kayaselcuk F, Ozer C, Oguzulgen I, Hasirci E
Department of Urology, Baskent University Adana Medical and Research Center, Adana, Turkey.
Department of Pathology, Baskent University Adana Medical and Research Center, Adana, Turkey.
Andrologia. 2017 Mar;49(2). doi: 10.1111/and.12614. Epub 2016 May 14.
This study was aimed to investigate the effects of experimental left varicocele (ELV) repair on hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expressions and angiogenesis in rat testis. ELVs were surgically created in 26 adult male Sprague-Dawley rats. Thirty days after surgery, ELV repair was performed in 13 of the rats. All rats subsequently underwent orchiectomy 30 days after the last laparotomy. Histology of ELV-repaired testicles was compared to that of the unrepaired (ELV) group. The frequency of positive HIF-1α findings was significantly lower in the ELV-repaired than in the ELV group. The frequency of positive VEGF findings was also lower in the ELV-repaired than in the ELV group, although the difference was not statistically significant (P = 0.238). The mean microvessel density in ELV-repair group was significantly lower than that in the ELV group (P = 0.002). Our study demonstrated that ELV repair may protect tissues from hypoxia and hypoxia-related pathophysiologic events, such as angiogenesis, in rat testis.
本研究旨在探讨实验性左侧精索静脉曲张（ELV）修复对大鼠睾丸中缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）表达以及血管生成的影响。对26只成年雄性Sprague-Dawley大鼠进行手术创建ELV。术后30天，对其中13只大鼠进行ELV修复。所有大鼠在最后一次剖腹手术后30天随后接受睾丸切除术。将ELV修复后的睾丸组织学与未修复（ELV）组进行比较。ELV修复组中HIF-1α阳性结果的频率显著低于ELV组。ELV修复组中VEGF阳性结果的频率也低于ELV组，尽管差异无统计学意义（P = 0.238）。ELV修复组的平均微血管密度显著低于ELV组（P = 0.002）。我们的研究表明，ELV修复可能保护大鼠睾丸组织免受缺氧以及与缺氧相关的病理生理事件，如血管生成的影响。
