Herbivorous Livestock Institute, Chongqing Academy of Animal Sciences, Chongqing, 402460, China.
Biotechnol Lett. 2020 Sep;42(9):1633-1644. doi: 10.1007/s10529-020-02906-0. Epub 2020 May 7.
MicroRNAs (miRNAs) are highly conserved, endogenous small RNAs that regulate gene expression at the post-transcriptional level. miR-127 plays an essential role in myogenic differentiation in vivo and in vitro. However, it is not clear whether miR-127-3p affects myogenic cell proliferation.
The detailed function of miR-127-3p in proliferative C2C12 cell lines and further identified its regulatory mechanism by qRT-PCR, western blot, flow cytometry analysis and luciferase reporter assay.
Overexpression of miR-127-3p significantly inhibited proliferation of C2C12 cells and vice versa. Sept7 was a target gene of miR-127-3p using dual-luciferase reporter assay, qRT-PCR, and western blotting. The RNA interference analysis, in which Sept7 was downregulated, showed that Sept7 significantly promoted the proliferation of C2C12 cells. Besides, the expression level of Sept7 was detected analysis in muscle cells and tissues.
These findings reveal that miR-127-3p regulates myoblast proliferation by targeting Sept7.
微小 RNA（miRNAs）是高度保守的内源性小 RNA，可在转录后水平调节基因表达。miR-127 在体内和体外的肌生成分化中起着至关重要的作用。然而，miR-127-3p 是否影响肌细胞增殖尚不清楚。
通过 qRT-PCR、western blot、流式细胞术分析和荧光素酶报告基因检测，详细研究了 miR-127-3p 在增殖性 C2C12 细胞系中的作用，并进一步鉴定了其调控机制。
miR-127-3p 的过表达显著抑制了 C2C12 细胞的增殖，反之亦然。双荧光素酶报告基因检测、qRT-PCR 和 western blot 显示，Sept7 是 miR-127-3p 的靶基因。RNA 干扰分析显示，下调 Sept7 可显著促进 C2C12 细胞的增殖。此外，还检测分析了肌肉细胞和组织中 Sept7 的表达水平。
这些发现表明，miR-127-3p 通过靶向 Sept7 调节成肌细胞的增殖。
