Effects of the () Gene on the Proliferation and Differentiation of Bovine Intramuscular Preadipocyte and 3T3-L1 Cells.

Intramuscular fat content and marbling affecting meat quality are important economic traits in beef cattle. (cell division cycle 10 or ), a member of the septin family involved in cellular proliferation, was considered as a functional and positional candidate gene for beef marbling. In a previous study, we revealed that the expression levels of were also positively correlated with marbling scores in Japanese Black cattle. However, the regulatory mechanism of the gene on IMF deposition in cattle remains unclear. In the present study, flow cytometry, EdU proliferation assays, and Oil Red O staining results showed that overexpression of could promote the differentiation of bovine intramuscular preadipocyte (BIMP) and 3T3-L1 cells, whereas knockdown of resulted in the opposite consequences. Furthermore, quantitative PCR and Western blotting results showed that overexpression of could promote the expression levels of adipogenic marker genes and / at both mRNA and protein levels in BIMP and 3T3-L1 cells, whereas knockdown of resulted in the opposite consequences. Our results provide new insights into the regulatory roles of in adipocytes in animals.