Cheng Zixuan, Li Xihe, Bao Siqin, Yamada Takahisa, Cao Guifang, Liu Jianfeng, Chen Aorigele, Tong Bin
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
Department of Agrobiology, Faculty of Agriculture, Niigata University, Niigata 950-2181, Japan.
Animals (Basel). 2023 Feb 9;13(4):609. doi: 10.3390/ani13040609.
Intramuscular fat content and marbling affecting meat quality are important economic traits in beef cattle. (cell division cycle 10 or ), a member of the septin family involved in cellular proliferation, was considered as a functional and positional candidate gene for beef marbling. In a previous study, we revealed that the expression levels of were also positively correlated with marbling scores in Japanese Black cattle. However, the regulatory mechanism of the gene on IMF deposition in cattle remains unclear. In the present study, flow cytometry, EdU proliferation assays, and Oil Red O staining results showed that overexpression of could promote the differentiation of bovine intramuscular preadipocyte (BIMP) and 3T3-L1 cells, whereas knockdown of resulted in the opposite consequences. Furthermore, quantitative PCR and Western blotting results showed that overexpression of could promote the expression levels of adipogenic marker genes and / at both mRNA and protein levels in BIMP and 3T3-L1 cells, whereas knockdown of resulted in the opposite consequences. Our results provide new insights into the regulatory roles of in adipocytes in animals.
影响肉质的肌内脂肪含量和大理石花纹是肉牛重要的经济性状。细胞分裂周期蛋白10（CDC10）是参与细胞增殖的septin家族成员，被认为是牛肉大理石花纹的功能和位置候选基因。在先前的研究中，我们发现CDC10的表达水平也与日本黑牛的大理石花纹评分呈正相关。然而，CDC10基因对牛肌内脂肪（IMF）沉积的调控机制仍不清楚。在本研究中，流式细胞术、EdU增殖试验和油红O染色结果表明，CDC10的过表达可促进牛肌内前体脂肪细胞（BIMP）和3T3-L1细胞的分化，而CDC10的敲低则产生相反的结果。此外，定量PCR和蛋白质印迹结果表明，CDC10的过表达可促进BIMP和3T3-L1细胞中脂肪生成标记基因PPARγ和C/EBPα在mRNA和蛋白质水平的表达，而CDC10的敲低则产生相反的结果。我们的研究结果为CDC10在动物脂肪细胞中的调控作用提供了新的见解。
