von Essen R, Lehtinen M V
Rheumatism Foundation Hospital, Heinola, Finland.
Scand J Rheumatol Suppl. 1988;75:46-9. doi: 10.3109/03009748809096739.
RF has been on the national laboratory quality control program since 1981. A pilot study had showed that most participants would be small laboratories performing qualitative slide latex tests only. Since we nevertheless wanted to be able to extrapolate test sensitivity data for each laboratory, we have sent out sets of 5-6 coded samples that in fact constitute a double dilution titration series. RF positive sera were pooled and the material calibrated with the WHO RF standard. The samples have contained 5-160 IU RF/ml and have been distributed once a year. The number of participants has varied from 58 to 104. Interlaboratory variation has diminished considerably since 1981. Inversions in the estimates of RF contents in the sample series have been notably rare. Waaler-Rose returns have mostly been too small to permit statistical evaluation. The same so far goes for the turbido- and nephelometric methods, in use since 1985.
自1981年以来，类风湿因子（RF）一直被纳入国家实验室质量控制项目。一项试点研究表明，大多数参与实验室为仅进行定性玻片乳胶试验的小型实验室。然而，由于我们希望能够推断出每个实验室的检测灵敏度数据，我们已发放了5 - 6组编码样本，这些样本实际上构成了一个双倍稀释滴定系列。将RF阳性血清混合，并使用世界卫生组织（WHO）的RF标准对材料进行校准。样本中RF含量为5 - 160 IU/ml，且每年分发一次。参与实验室的数量从58个到104个不等。自1981年以来，实验室间的差异已大幅减少。样本系列中RF含量估计值的倒置情况极为罕见。瓦勒 - 罗斯（Waaler - Rose）反应结果大多过小，无法进行统计评估。自1985年以来使用的比浊法和散射比浊法目前也是如此。
