Singer J M, Edberg S C, Selinger M, Amram M
Am J Clin Pathol. 1979 Oct;72(4):591-6. doi: 10.1093/ajcp/72.4.591.
Standardization of the latex-fixation test for the detection of rheumatoid factor may be achieved by the preparation of a standard reference serum. A number of guidelines for the quality control of precision and sensitivity of the test are suggested. In the use of dilution procedures, a 0.1 log10 or a 0.05 log10 difference between tubes is employed. The end point is defined and titer expressed in terms of a final dilution represented by the amount of antigen-antibody added. For statistical purposes all serologic data are geometrically expressed. Commercial kits may be standardized in terms of minimum detectable units and normalized for titer and unit values to enable laboratories to compare results.
类风湿因子检测乳胶凝集试验的标准化可通过制备标准参考血清来实现。文中提出了一些关于该试验精密度和灵敏度质量控制的指导原则。在使用稀释程序时，各管之间采用0.1 log10或0.05 log10的差异。定义终点，并根据添加的抗原抗体量所代表的最终稀释度来表示滴度。出于统计目的，所有血清学数据均以几何形式表示。商业试剂盒可根据最低检测单位进行标准化，并对滴度和单位值进行归一化处理，以便各实验室比较结果。
