Feltkamp T E
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Scand J Rheumatol Suppl. 1988;75:54-7. doi: 10.3109/03009748809096741.
About 1% of the Dutch population is per year checked for the first time for the presence of rheumatoid factors (RF). Unfortunately, the interlaboratory variations of the titres of RF tests are enormous. We therefore (re)studied the effect of comparisons of the results with the WHO standard rheumatoid serum, leading to expression of the results in international units (IU), instead of in titres. Testing 5 sera in duplicate in 33 laboratories, a significant decrease of the interlaboratory variability was noted for the Waaler-Rose test, the Latex fixation test and the ELISA for the demonstration of RF's. Also the results of the antiperinuclear factor (APF) test improved significantly. The results of RF's demonstrated with the indirect immunofluorescence technique showed already a satisfactory comparability. Expression in IU's had no significant effect for this test. The introduction of the use of a reference serum met no opposition. Nearly all Dutch laboratories express now their results in units.
每年约1%的荷兰人口首次接受类风湿因子（RF）检测。遗憾的是，RF检测结果在不同实验室间的差异极大。因此，我们重新研究了将结果与世界卫生组织标准类风湿血清进行比对的效果，从而使结果以国际单位（IU）而非滴度来表示。在33个实验室对5份血清进行双份检测，结果发现，对于瓦勒 - 罗斯试验、乳胶凝集试验以及用于检测RF的酶联免疫吸附测定（ELISA），实验室间变异性显著降低。抗核周因子（APF）检测结果也有显著改善。采用间接免疫荧光技术检测RF的结果已显示出令人满意的可比性。以IU表示对该检测无显著影响。引入使用参考血清未遭反对。现在几乎所有荷兰实验室都以单位来表示其结果。
