Institute of Medical Physiology "Richard Burian", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
University of Belgrade-School of Electrical Engineering, Belgrade, Serbia.
PLoS One. 2020 May 8;15(5):e0232530. doi: 10.1371/journal.pone.0232530. eCollection 2020.
Clinical and animal studies have found that anxiety and depression are significantly more common after acute myocardial infarction (AMI). The medial prefrontal cortex (PFC) has a dual role: in higher brain functions and in cardiovascular control, making it a logical candidate for explaining the perceived bidirectional heart-brain connection. We used parallel Electrocardiography (ECG) and Electrocorticography (ECoG) registration to investigate AMI-induced changes in medial PFC bioelectrical activity in a rat model of AMI.
Adult male Wistar albino rats were used in the study. Gold-plated recording electrodes were implanted over the frontal cortex for ECoG recording. ECG was recorded via two holter electrodes attached on the skin of the back fixed in place by a jacket. Induction of AMI was performed by isoprenaline (150 mg/kg, i.p.). ECoG and ECG signals were registered at baseline, during 3 hours after isoprenaline administration and at 24 hours after isoprenaline administration.
Significant increases of theta, alpha, and beta electroencephalographic (EEG) band power were observed in different time intervals after isoprenaline administration. Significant increase of theta band peak frequency was also observed during the first hour after isoprenaline administration. No statistically significant differences in band-power activity were found between the pre-isoprenaline measurements and 24 hours after administration.
Our results demonstrate significant increases in EEG band power of alpha beta and theta bands during isoprenaline-induced AMI model. These are the first findings to connect heart damage during isoprenaline- induced AMI to disturbances in the cortical bioelectrical activity.
临床和动物研究发现，急性心肌梗死（AMI）后焦虑和抑郁明显更为常见。内侧前额叶皮层（PFC）具有双重作用：在高级脑功能和心血管控制中，使其成为解释感知到的双向心脑连接的逻辑候选者。我们使用并行心电图（ECG）和皮层电图（ECoG）记录来研究 AMI 诱导的内侧 PFC 生物电活动变化在 AMI 大鼠模型中。
本研究使用成年雄性 Wistar 白化大鼠。金镀记录电极植入额皮质进行 ECoG 记录。ECG 通过两个 Holter 电极记录，这些电极通过固定在夹克上的背部皮肤上的两个 Holter 电极附着在皮肤上。通过异丙肾上腺素（150mg/kg，ip）诱导 AMI。在基线、异丙肾上腺素给药后 3 小时和异丙肾上腺素给药后 24 小时记录 ECoG 和 ECG 信号。
异丙肾上腺素给药后不同时间间隔观察到θ、α和β脑电（EEG）带功率的显著增加。异丙肾上腺素给药后第一小时也观察到θ带峰值频率的显著增加。在异丙肾上腺素给药前的测量值和给药后 24 小时之间，带功率活动没有统计学上的显著差异。
我们的结果表明，在异丙肾上腺素诱导的 AMI 模型中，α、β和θ 波段的 EEG 带功率显著增加。这些是首次将异丙肾上腺素诱导的 AMI 期间的心脏损伤与皮质生物电活动紊乱联系起来的发现。
