Lane E M, Ramaley R F, Miller N G
Department of Medical Microbiology, University of Nebraska College of Medicine, Omaha 68105.
Southeast Asian J Trop Med Public Health. 1988 Dec;19(4):629-32.
Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium with hemin. The hemolysin produced in this culture system showed a greatly expanded spectrum of hemolysis as compared to previous reports of leptospiral hemolysin produced in a more traditional culture system containing serum.