Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Łódź University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland.
Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland.
Biomolecules. 2020 May 6;10(5):724. doi: 10.3390/biom10050724.
An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial strains, whereas pathogenic bacteria and yeast (), responsible for infections in breeding animals, showed much weaker adhesion.
采用酶法和微生物介导的生物质转化，从糖甜菜渣（SBP）中释放果胶衍生低聚糖（POS）的有效且生态的方法已经开发出来。POS 可应用于生产益生元饲料添加剂。筛选了各种酵母菌株，以评估其蛋白质合成和单糖同化能力。发现酵母共培养和果胶水解是生产益生元的有效方法。单独的酶解和 SBP 发酵分别导致每 100g d.w. 释放 3.6g POS，而经组合工艺获得的 POS 产量高出 17.9%，每 100g d.w. 可获得 4.2g POS。由于单糖和二糖对酶的抑制作用降低，将酵母引入该过程可提高水解性能。通过使用胃肠道细菌的单独培养物进行发酵，评估了 POS 的益生元作用。SBP 水解物中的 POS 可有效促进乳酸菌和双歧杆菌的生长。在存在 POS 的情况下，有益菌株对 Caco-2 细胞的黏附显著增加，而负责养殖动物感染的病原菌和酵母（）的黏附则弱得多。
