Wilkowska Agnieszka, Nowak Adriana, Motyl Ilona, Oracz Joanna
Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Łódź University of Technology, Wólczańska 171/173, 90-530 Łódź, Poland.
Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-530 Łódź, Poland.
Molecules. 2024 Dec 26;30(1):46. doi: 10.3390/molecules30010046.
The purpose of this research was to investigate the prebiotic effects of different fractions of pectin-derived oligosaccharides (POSs) from apple pomace (AP) in relation to their molecular weight (MW), structure, and composition. Enzymatic treatment of the apple pomace resulted in high-molecular-weight arabinans and rhamnogalacturonans (MW 30-100 kDa, MW 10-30 kDa), as well as oligomeric fractions with molecular weights of less than 10 kDa, consisting mainly of homogalacturonan. The biological potential of the POSs against various lactobacilli and bifidobacteria was evaluated. The oligosaccharides with the highest molecular weights (MW 30-100 kDa, MW 10-30 kDa) showed better prebiotic effect to lactobacilli. The oligosaccharides with MW 3-10 kDa and MW 10-30 kDa caused an increase in the bifidogenic effect. Inhibition of , serovar Typhimurium, and was also observed. The preparations with MW 3-10 kDa and MW 10-30 kDa demonstrated the strongest biological activity, supporting the adhesion of beneficial microorganisms to mucin and collagen surfaces. Therefore, oligosaccharides with MW 10-30 kDa were considered to be the most promising prebiotic candidates. This study confirms that the biological effects of pectic oligosaccharides vary significantly based on their structural differences. Therefore, the conditions of enzymatic hydrolysis of apple pectin should be optimized to obtain oligosaccharides within a specific molecular mass range.
本研究的目的是调查苹果渣（AP）中不同级分的果胶衍生寡糖（POSs）的益生元效应，及其分子量（MW）、结构和组成的关系。对苹果渣进行酶处理，得到了高分子量的阿拉伯聚糖和鼠李糖半乳糖醛酸聚糖（分子量30 - 100 kDa，分子量10 - 30 kDa），以及分子量小于10 kDa的寡聚级分，主要由同型半乳糖醛酸聚糖组成。评估了POSs对各种乳酸杆菌和双歧杆菌的生物学潜力。分子量最高的寡糖（分子量30 - 100 kDa，分子量10 - 30 kDa）对乳酸杆菌显示出更好的益生元效应。分子量为3 - 10 kDa和10 - 30 kDa的寡糖导致双歧杆菌生成效应增加。还观察到对鼠伤寒沙门氏菌血清型和的抑制作用。分子量为3 - 10 kDa和10 - 30 kDa的制剂表现出最强的生物活性，支持有益微生物在粘蛋白和胶原蛋白表面的粘附。因此，分子量为10 - 30 kDa的寡糖被认为是最有前途的益生元候选物。本研究证实，果胶寡糖的生物学效应因其结构差异而有显著不同。因此，应优化苹果果胶的酶水解条件，以获得特定分子量范围内的寡糖。
