Wiese Maria, Hui Yan, Holck Jesper, Sejberg Jimmy J P, Daures Celia, Maas Evy, Kot Witold, Borné Johanna M, Khakimov Bekzod, Thymann Thomas, Nielsen Dennis Sandris
CP Kelco ApS, Lille Skensved, Denmark.
Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg C, Denmark.
Anim Microbiome. 2021 Oct 9;3(1):69. doi: 10.1186/s42523-021-00129-w.
Fiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use.
We screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure-function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA).
Sugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS.
Our results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients.
富含纤维的饲料成分具有益生元潜力，可增强猪的健康，在抗生素和氧化锌使用分阶段暂停的情况下，被视为解决养猪生产中断奶后腹泻高发病率问题的潜在方案。
我们使用体外结肠模型CoMiniGut，在刚断奶仔猪的肠道微生物群中筛选了由甜菜制备的果胶底物对肠道微生物群的调节特性。我们专注于测试13种具有明确结构的甜菜衍生果胶底物，以及已知的益生元，如菊粉、低聚果糖（FOS）和低聚半乳糖（GOS），以深入了解特定底物对断奶仔猪肠道微生物组成以及短链脂肪酸产生（SCFA）的结构-功能相关特性。
甜菜衍生的果胶和鼠李糖半乳糖醛酸聚糖-I选择性地增加了拟杆菌门的相对丰度，特别是Copri普雷沃氏菌、卵形拟杆菌、酸化拟杆菌和一个未分类的拟杆菌成员。酯化程度影响了这些物种的相对丰度以及体外发酵过程中的SCFA产生。源自甜菜的改性阿拉伯聚糖促进了Blautia、Copri普雷沃氏菌、毛螺菌科成员和粘膜利莫西尔乳杆菌的生长，并且在所有测试的低聚糖中，发酵24小时后产生的总SCFA量最高。相对于已知的益生元菊粉、FOS和GOS，甜菜衍生的底物产生了更高的总SCFA浓度（尤其是乙酸和丙酸）。
我们的结果表明，仅从一种植物来源（甜菜）制备的果胶的分子结构可以选择性地刺激不同的肠道微生物群成员，突出了利用果胶底物作为靶向肠道微生物群调节成分的潜力。
