Department of Medical Imaging, Shandong Provincial Third Hospital, Jinan 250031, Shandong, PR China.
Department of Ultrasonography, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical Univerisity, Jinan 250014, Shandong, PR China.
J Nanosci Nanotechnol. 2020 Oct 1;20(10):6561-6567. doi: 10.1166/jnn.2020.18437.
This study aimed to determine the effects of dimer captosuccinic acid-coated Fe₃O₄ (super paramagnetic) nanoparticles (NP) on 2-deoxy-d-glucose in targeted cancer cells with high rates of glucose metabolism. We prepared Fe₃O₄@DMSA NP and 2-DG-conjugated Fe₃O₄@DMSA NP, -FE, O, and @DMSA-DG NP. Glucose consumption in MDA-MB-231 and MCF-7 breast cancer cells was determined using -Fe2O3@DMSA NP or Fe₃O₄@DMSA-DG NP, and absorption was tested using Prussian blue staining, ultraviolet colorimetry, and magnetic resonance imaging. Glucose consumption was the highest in MDA-MB-231, and the lowest in human mammary epithelial cells (HMEPiC). The significant uptake of Fe2O3@DMSA-DG NP by MDA-MB-231 and MCF-7 cells within two hours was inhibited by glucose. The uptake of Fe₃O₄@DMSA-DG NP was significantly higher in MDA-MB-231 than in MCF-7 cells, whereas Fe₃O₄@DMSA NP was not obviously uptaken by either cell line. Absorption was also not evident in HMEPiC incubated with Fe₃O₄@DMSA-DG NP and Fe₃O₄@DMSA NP. The tumor targeting efficacy of 2-DG coated Fe₃O₄@DMSA NP was improved over Fe₃O₄,@DMSA NP in cancer cells with high rates of glucose metabolism.
本研究旨在确定二聚体琥珀酸酐涂层 Fe₃O₄（超顺磁性）纳米粒子（NP）对葡萄糖代谢率高的靶向癌细胞中 2-脱氧-d-葡萄糖的影响。我们制备了 Fe₃O₄@DMSA NP 和 2-DG 偶联的 Fe₃O₄@DMSA NP、-FE、O 和@DMSA-DG NP。用 -Fe2O3@DMSA NP 或 Fe₃O₄@DMSA-DG NP 测定 MDA-MB-231 和 MCF-7 乳腺癌细胞的葡萄糖消耗，并通过普鲁士蓝染色、紫外比色法和磁共振成像测试吸收。MDA-MB-231 的葡萄糖消耗最高，人乳腺上皮细胞（HMEPiC）的葡萄糖消耗最低。MDA-MB-231 和 MCF-7 细胞在两小时内对 Fe2O3@DMSA-DG NP 的摄取显著受到葡萄糖的抑制。MDA-MB-231 细胞对 Fe₃O₄@DMSA-DG NP 的摄取明显高于 MCF-7 细胞，而两种细胞系均未明显摄取 Fe₃O₄@DMSA NP。用 Fe₃O₄@DMSA-DG NP 和 Fe₃O₄@DMSA NP 孵育 HMEPiC 也没有明显的吸收。与 Fe₃O₄,@DMSA NP 相比，2-DG 包被的 Fe₃O₄@DMSA NP 在葡萄糖代谢率高的癌细胞中的肿瘤靶向效果得到改善。
