Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch, South Africa.
J Ind Microbiol Biotechnol. 2020 May;47(4-5):357-372. doi: 10.1007/s10295-020-02280-w. Epub 2020 May 8.
Yeast and microalgae are microorganisms with widely diverging physiological and biotechnological properties. Accordingly, their fields of applications diverge: yeasts are primarily applied in processes related to fermentation, while microalgae are used for the production of high-value metabolites and green technologies such as carbon capture. Heterotrophic-autotrophic systems and synthetic ecology approaches have been proposed as tools to achieve stable combinations of such evolutionarily unrelated species. We describe an entirely novel synthetic ecology-based approach to evolve co-operative behaviour between winery wastewater isolates of the yeast Saccharomyces cerevisiae and microalga Chlorella sorokiniana. The data show that biomass production and mutualistic growth improved when co-evolved yeast and microalgae strains were paired together. Combinations of co-evolved strains displayed a range of phenotypes, including differences in amino acid profiles. Taken together, the results demonstrate that biotic selection pressures can lead to improved mutualistic growth phenotypes over relatively short time periods.
酵母和微藻是具有广泛生理和生物技术特性的微生物。因此，它们的应用领域也不同：酵母主要应用于与发酵相关的过程，而微藻则用于生产高价值代谢物和绿色技术，如碳捕获。异养-自养系统和合成生态学方法已被提议作为实现这种进化上无关物种稳定组合的工具。我们描述了一种全新的基于合成生态学的方法，用于在酿酒厂废水中分离的酵母酿酒酵母和微藻绿藻之间进化出合作行为。数据表明，当共进化的酵母和微藻菌株配对时，生物量的产生和互利生长得到了改善。共进化菌株的组合表现出多种表型，包括氨基酸谱的差异。总的来说，这些结果表明，生物选择压力可以在相对较短的时间内导致互利生长表型的改善。
