Nisenbom H E, Perazzo J C, Monserrat A J, Vidal J C
Instituto de Neurobiología (IDNEU), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Toxicon. 1988;26(12):1137-44. doi: 10.1016/0041-0101(88)90298-x.
The effects on lethal potency and enzymatic activity were determined following alkylation, with p-bromophenacyl bromide, of the acidic toxic phospholipase A2 from Bothrops alternatus. The modified B. alternatus enzyme, which lost its enzymatic activity, retained considerable toxicity. Histopathologic studies on mice have demonstrated features similar to those of the native enzyme. However, the distribution of the damage was different and the survival time was longer. It is concluded that the enzyme activity is not important for the lethal action of the enzyme although it influences the distribution of the damage and survival time.
用对溴苯甲酰溴对变色矛头蝮的酸性有毒磷脂酶A2进行烷基化处理后，测定了其对致死效力和酶活性的影响。经过修饰的变色矛头蝮酶失去了酶活性，但仍保留了相当大的毒性。对小鼠的组织病理学研究表明，其特征与天然酶相似。然而，损伤的分布不同，存活时间更长。得出的结论是，酶活性对该酶的致死作用并不重要，尽管它会影响损伤的分布和存活时间。
