Chwetzoff S, Ménez A
Service de Biochimie, C.E.N.-Saclay, Gif-sur-Yvette.
C R Acad Sci III. 1988;307(2):29-32.
Nigexine is a basic phospholipase A2 isolated from the venom of Naja nigricollis (Institut Pasteur). Previous works have shown that nigexine is (i) cytotoxic in vitro toward epithelial cells of FL strain and, (ii) toxic to mice (LD50 = 29 nmoles/kg). We have investigated the properties of nigexine treated with p-bromophenacyl bromide, a reagent which specifically destroys the enzymatic activity of phospholipases A2. We report that pure derivatized nigexine has no lethal detectable activity to mice whereas it still possesses a residual 20% cytotoxic activity, as compared with the parental compound.
黑眼镜蛇毒磷脂酶A2是从黑颈眼镜蛇（巴斯德研究所）毒液中分离出的一种碱性磷脂酶A2。以往的研究表明，黑眼镜蛇毒磷脂酶A2（i）在体外对FL株上皮细胞具有细胞毒性，（ii）对小鼠有毒性（半数致死量=29纳摩尔/千克）。我们研究了用对溴苯甲酰溴处理的黑眼镜蛇毒磷脂酶A2的特性，对溴苯甲酰溴是一种能特异性破坏磷脂酶A2酶活性的试剂。我们报告称，纯衍生化黑眼镜蛇毒磷脂酶A2对小鼠没有可检测到的致死活性，而与亲本化合物相比，它仍具有20%的残余细胞毒性活性。
