Department of Genetics, Microbiology and Statistics, University of Barcelona, Avda/ Diagonal 643, 08028 Barcelona, Spain; The Water Research Institute, University of Barcelona, C/ Montalegre 6, 08001 Barcelona, Spain.
Department of Genetics, Microbiology and Statistics, University of Barcelona, Avda/ Diagonal 643, 08028 Barcelona, Spain; The Water Research Institute, University of Barcelona, C/ Montalegre 6, 08001 Barcelona, Spain.
J Microbiol Methods. 2020 Jun;173:105940. doi: 10.1016/j.mimet.2020.105940. Epub 2020 May 5.
Somatic and F-specific coliphages are gaining ground as indicators of fecal/viral pollution. Guidelines and regulations worldwide for monitoring water, biosolids and food are including them as parameters to assess quality and treatment efficiency. Robust methods to detect and quantify both groups of phages in water samples have been launched by agencies such as the International Standardization Organization (ISO) and the USA Environmental Protection Agency (USEPA). Although these methods have proved readily implementable in routine microbiology laboratories, faster and more user-friendly protocols will be highly welcome if coliphage detection becomes routine in water quality analysis. We here provide an overview of new approaches seeking to facilitate the detection of infectious coliphages included in guidelines and regulations. The improvements achieved suggest that streamlined kits able to provide results in a few hours at very reasonable costs will become available in the near future. The potential of molecular procedures and methods based on microelectronic sensors is also briefly discussed.
肠道和 F 型噬菌体作为粪便/病毒污染的指示物正在逐渐得到认可。全球范围内用于监测水、生物固体和食品的指南和法规将它们作为评估质量和处理效率的参数。国际标准化组织（ISO）和美国环境保护署（USEPA）等机构已经推出了检测水样中这两组噬菌体的强大方法。尽管这些方法已被证明可在常规微生物学实验室中轻松实施，但如果在水质分析中常规检测噬菌体，更快、更用户友好的协议将受到高度欢迎。在这里，我们概述了旨在促进指南和法规中包含的传染性噬菌体检测的新方法。所取得的改进表明，能够在几个小时内以非常合理的成本提供结果的简化试剂盒将在不久的将来问世。还简要讨论了基于分子程序和微电子传感器的方法的潜力。
