School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China; State Key Laboratory of Freshwater Ecology and Biotechnology, and Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, and Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China.
Fish Shellfish Immunol. 2020 Jul;102:326-335. doi: 10.1016/j.fsi.2020.04.069. Epub 2020 May 5.
In fish, type I IFNs are classified into three groups, i.e. group one, group two and group three, and further separated into seven subgroups based on the number of conserved cysteines and phylogenetic relationships. In the present study, four type I IFNs, named as IFNϕ1, IFNϕ2, IFNϕ3, IFNϕ4, as reported in zebrafish, were identified in a cyprinid, the topmouth culter, Culter alburnus, a species introduced recently into China's aquaculture. These IFNs may be classified as IFNa, IFNc, IFNc and IFNd in a recent nomenclature, with IFNa and IFNd having two cysteines in group one, and IFNc four cysteines in group two. These IFNs, together with their possible receptors, IFNϕ1, IFNϕ2, IFNϕ3, IFNϕ4, and CRFB1, CRFB2 and CRFB5 have an open reading frame (ORF) of 540, 552, 567, 516 bp, and 1572, 1392, 1125 bp, respectively. These IFNs have high amino acid sequence identities, being 91.1-93.6% and 66.9-77.3%, with those in grass carp and zebrafish, respectively, and are expressed constitutively in organs/tissues examined in the fish. The expression of these IFNs can be further induced following poly (I:C) stimulation. However, the possible function of these IFNs and their signalling pathway are of interest for further research.
在鱼类中，I 型干扰素分为三组，即一组、二组和三组，并根据保守半胱氨酸的数量和系统发育关系进一步分为七个亚组。在本研究中，在一种引入中国水产养殖的鲤科鱼类翘嘴红鲌中鉴定到了四种 I 型干扰素，命名为 IFNϕ1、IFNϕ2、IFNϕ3 和 IFNϕ4。根据最近的命名法，这些 IFN 可归类为 IFNa、IFNc、IFNc 和 IFNd，其中 IFNa 和 IFNd 在一组中有两个半胱氨酸，IFNc 在二组中有四个半胱氨酸。这些 IFN 及其可能的受体 IFNϕ1、IFNϕ2、IFNϕ3、IFNϕ4、CRFB1、CRFB2 和 CRFB5 的开放阅读框（ORF）分别为 540、552、567、516bp 和 1572、1392、1125bp。这些 IFN 具有高度的氨基酸序列同一性，与草鱼和斑马鱼的同源性分别为 91.1-93.6%和 66.9-77.3%，并在检测的鱼类器官/组织中组成性表达。这些 IFN 的表达可以在多聚（I:C）刺激后进一步诱导。然而，这些 IFN 的可能功能及其信号通路值得进一步研究。
