斑马鱼中膜孕激素受体（mPR）敲除导致卵母细胞成熟和排卵受损。
Impaired oocyte maturation and ovulation in membrane progestin receptor (mPR) knockouts in zebrafish.
机构信息
Department of Biology, East Carolina University, Greenville, NC, USA.
Department of Biology, East Carolina University, Greenville, NC, USA; College of Ocean and Earth Sciences, Xiamen University, Fujian, 361005, PR China.
出版信息
Mol Cell Endocrinol. 2020 Jul 1;511:110856. doi: 10.1016/j.mce.2020.110856. Epub 2020 May 5.
Abstract
Accumulating evidence suggest that membrane progestin receptor α (mPRα) is the membrane receptor mediating nongenomic progestin signaling that induces oocyte maturation in teleost. However, the involvement of other members of mPR family in oocyte maturation is still unclear. In this study, we found impaired oocyte maturation in zebrafish lacking mPRα1, mPRα2, mPRβ, or mPRγ2. In contrast, no difference was observed in oocyte maturation in the single knockout of mPRγ1, mPRδ, or mPRε. To study possible redundant functions of different mPRs in oocyte maturation, we generated a zebrafish line lacking all seven kinds of mPRs (mprs). We found oocyte maturation was further impaired in mprs. In addition, oocyte ovulation delay was observed in mprs females, which was associated with low levels of nuclear progestin receptor (Pgr), a key regulator for ovulation. We also found reduced fertility in mprs female zebrafish. Furthermore, eggs spawned by mprs females were of poor quality.
摘要
越来越多的证据表明，膜孕激素受体 α（mPRα）是介导非基因组孕激素信号转导的膜受体，该信号转导诱导鱼类卵母细胞成熟。然而，mPR 家族的其他成员是否参与卵母细胞成熟尚不清楚。在这项研究中，我们发现 mPRα1、mPRα2、mPRβ 和 mPRγ2 缺失的斑马鱼卵母细胞成熟受损。相比之下，mPRγ1、mPRδ 或 mPRε 缺失的单敲除斑马鱼卵母细胞成熟没有差异。为了研究不同 mPR 在卵母细胞成熟中可能存在的冗余功能，我们生成了一种缺乏所有七种 mPR 的斑马鱼系（mprs）。我们发现 mprs 中的卵母细胞成熟进一步受损。此外，mprs 雌性出现卵母细胞排卵延迟，这与排卵的关键调节因子核孕激素受体（Pgr）水平降低有关。我们还发现 mprs 雌性斑马鱼的生育能力降低。此外，mprs 雌性产卵的质量较差。
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