Vietnam Research Station, Center for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
Bacteriology Department, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
J Glob Antimicrob Resist. 2020 Jun;21:272-274. doi: 10.1016/j.jgar.2020.04.033. Epub 2020 May 6.
The aim of this study was to understand the natural bacterial hosts of antimicrobial resistance genes (ARGs) and their impact on the processes of evolution, spread and positive selection of acquired ARGs.
Environmental carbapenem-resistant Gram-negative bacteria in Vietnam were screened for based on a One Health approach. Whole-genome sequencing (WGS) and comparative genomic analysis of the isolates were performed. WGS of three carbapenem-resistant Shewanella xiamenensis strains (SxND_W2_2018, SxND_W5_2018 and SxND_W9_2018) isolated from canals in Truc Ninh District and Nghia Hung District, Nam Dinh Province, Vietnam, in 2018 was performed using an Illumina MiniSeq system. ARGs in the draft genome sequences were detected using ResFinder, and comparison of genomic regions was performed using BLASTn and Easyfig.
Thebla-like carbapenem-hydrolysing class D β-lactamase genes bla, bla and bla were detected in chromosomal contigs of SxND_W2_2018, SxND_W5_2018 and SxND_W9_2018, respectively. Comparative analysis of the surrounding regions of the bla-like genes, including both 10 kb upstream and 10 kb downstream of the genes, showed that the genomic regions were highly conserved in all three isolates.
This study analysed the draft genome sequences of carbapenem-resistantS. xiamenensis strains isolated from a water environment in Vietnam. All of the strains carried bla-like gene variants in their chromosomes. This information will contribute to highlight the evolution of bla family carbapenemase genes in nature and the importance of S. xiamenensis as a natural reservoir of important ARGs in the environment in Vietnam.
本研究旨在了解抗微生物药物耐药基因（ARGs）的天然细菌宿主及其对获得性 ARGs 的进化、传播和正选择过程的影响。
采用“One Health”方法筛选越南环境中耐碳青霉烯类的革兰氏阴性细菌。对分离株进行全基因组测序（WGS）和比较基因组分析。对 2018 年从越南南定省翠宁区和义安区运河中分离的三株耐碳青霉烯类厦门希瓦氏菌（SxND_W2_2018、SxND_W5_2018 和 SxND_W9_2018）菌株进行 WGS，使用 Illumina MiniSeq 系统。使用 ResFinder 检测基因组草案序列中的 ARGs，使用 BLASTn 和 Easyfig 进行基因组区域比较。
在 SxND_W2_2018、SxND_W5_2018 和 SxND_W9_2018 的染色体连续体中分别检测到 bla 样碳青霉烯水解类 Dβ-内酰胺酶基因 bla、bla 和 bla。对 bla 样基因周围区域的比较分析，包括基因上下游 10kb 的区域，显示三个分离株的基因组区域高度保守。
本研究分析了从越南水环境中分离的耐碳青霉烯类厦门希瓦氏菌菌株的基因组草案序列。所有菌株的染色体上都携带 bla 样基因变体。这些信息将有助于突出 bla 家族碳青霉烯酶基因在自然界中的进化以及厦门希瓦氏菌作为越南环境中重要 ARGs 天然储库的重要性。
