LuxS/AI-2群体感应系统调控Lzh-2的杀藻活性。
The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Lzh-2.
作者信息
Liu Jian, Liu Kaiquan, Zhao Zhe, Wang Zheng, Wang Fengchao, Xin Yuxiu, Qu Jie, Song Feng, Li Zhenghua
机构信息
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, China.
School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China.
出版信息
Front Microbiol. 2022 Jan 28;12:814929. doi: 10.3389/fmicb.2021.814929. eCollection 2021.
Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system.
蓝藻水华是一个在全球范围内日益严重的环境问题。杀藻细菌是控制水华的潜在工具。许多细菌中的杀藻活性表现出群体感应（QS）动态，而这些细菌中这种活性的调控机制尚不清楚。在本研究中，通过结合基因组测序和基因组编辑，我们确定了分离出的杀藻菌株Lzh-2中的主要群体感应系统是LuxS/AI-2信号通路。通过重组缺失LuxS基因破坏QS系统导致杀藻剂产生和杀藻活性丧失。在缺失突变体中恢复LuxS基因可补偿QS系统并恢复杀藻活性。因此，我们证明Lzh-2通过LuxS/AI-2群体感应系统调节杀藻活性。
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