植物单细胞基因组学的前景。
The promise of single-cell genomics in plants.
机构信息
Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Department of Genome Sciences, University of Washington, Seattle, WA, USA; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA.
出版信息
Curr Opin Plant Biol. 2020 Apr;54:114-121. doi: 10.1016/j.pbi.2020.04.002. Epub 2020 May 5.
Abstract
Single-cell genomic approaches have the potential to revolutionize the study of plant systems. Here, we highlight newly developed techniques to analyze transcriptomes at single-cell resolution. We focus on the rigorous standards necessary to generate and compare these data sets introducing analysis methods that can be applied to interpret their results. Lastly, we discuss the inherent limitations of single-cell studies and address future directions for plant single-cell genomics.
摘要
单细胞基因组学方法有可能彻底改变植物系统的研究。在这里，我们重点介绍新开发的技术，以单细胞分辨率分析转录组。我们专注于生成和比较这些数据集所需的严格标准，介绍可用于解释其结果的分析方法。最后，我们讨论了单细胞研究的固有局限性，并为植物单细胞基因组学的未来发展方向提出了建议。
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