School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 5;238:118412. doi: 10.1016/j.saa.2020.118412. Epub 2020 Apr 28.
Ascorbic acid (AA) is an important diet-derived antioxidant to human body. Thus, efficient and accurate detection of AA is of considerable significance in food analysis. Herein, smartphone assisted colorimetric and fluorescent triple-channel signal sensor has been developed for AA monitoring based on oxidase-like CoOOH nanoflakes. CoOOH nanoflakes can efficiently catalyze the oxidation of p-phenylenediamine (p-PD) into reddish brown p-PDox. The carbon dots (C-dots) are further introduced, of which the fluorescence can be quenched by p-PDox. However, in the presence of AA, the CoOOH nanoflakes is reduced and thus collapsed. As a result, the oxidation of p-PD is restrained, and thus the fluorescence of C-dots keeps strong. Based on AA induced light color, low absorbance, and strong fluorescence, triple-channel signal sensor has been proposed for AA determination. The AA assay shows a dynamic response range from 0.5 to 10 μM with a detection limit of 0.09 μM. The method assay allows detection of AA in real samples such as fruit juices. Combination with portable smartphone, the developed sensor is potential for AA determination in resource-poor settings.
抗坏血酸（AA）是人体重要的饮食来源抗氧化剂。因此，高效、准确地检测 AA 在食品分析中具有重要意义。本文基于类氧化酶 CoOOH 纳米片，开发了智能手机辅助比色和荧光三通道信号传感器，用于 AA 的监测。CoOOH 纳米片可以有效地催化对苯二胺（p-PD）氧化生成红棕色的 p-PDox。进一步引入了碳点（C-dots），其荧光可以被 p-PDox 猝灭。然而，在 AA 的存在下，CoOOH 纳米片被还原并因此坍塌。结果，p-PD 的氧化受到抑制，因此 C-dots 的荧光保持较强。基于 AA 诱导的光色、低吸光度和强荧光，提出了三通道信号传感器用于 AA 的测定。AA 的测定范围为 0.5 到 10 μM，检测限为 0.09 μM。该方法可用于检测果汁等实际样品中的 AA。与便携式智能手机相结合，该传感器有望用于资源匮乏环境中的 AA 测定。
