Ikegami M, Morinaga T, Miura K
Nodai Research Institute, Tokyo University of Agriculture.
Virus Genes. 1988 Mar;1(2):191-203. doi: 10.1007/BF00555937.
Comparison of the nucleotide sequences of the DNAs of bean golden mosaic virus (BGMV), tomato golden mosaic virus (TGMV) and cassava latent virus (CLV) revealed a fairly close relationship between BGMV DNA1, TGMV DNA1, and CLV DNA1 and a comparatively distant relationship between BGMV DNA2, TGMV DNA2, and CLV DNA2. The 200-base region common to the two DNAs of each virus had little sequence homology, except for a highly conserved 33-36 base sequence potentially capable of forming a stable hairpin structure. All the potential coding regions in the BGMV DNAs had counterparts in the TGMV and CLV DNAs suggesting an overall similarity in genome organization but two potential coding regions in the BGMV DNAs had no counterparts in the TGMV DNAs. The most highly conserved ORFs, BGMV 1R1, TGMV 1R1, and CLV 1R1, are the putative genes for the coat proteins of BGMV, TGMV, and CLV. BGMV 1R1 has 91.9% and 71.6% homology with respect to TGMV and CLV. The ORFs (BGMV 1L1; CLV 1L1; TGMV 1L1) and the two smaller overlapping ORFs (BGMV 1L2, 1L3; TGMV 1L2, 1L3; CLV 1L5, 1L3) are conserved in the three viruses. BGMV 2R1 and BGMV 2L1 have higher homology with respect to TGMV but not with respect to 2R1 and 2L1 in CLV. From this study we conclude that BGMV is more closely related to TGMV than CLV.
对菜豆金色花叶病毒（BGMV）、番茄金色花叶病毒（TGMV）和木薯潜伏病毒（CLV）的DNA核苷酸序列进行比较后发现，BGMV DNA1、TGMV DNA1和CLV DNA1之间关系相当密切，而BGMV DNA2、TGMV DNA2和CLV DNA2之间的关系则相对较远。每种病毒的两条DNA共有的200个碱基区域几乎没有序列同源性，只有一段高度保守的33 - 36个碱基的序列，可能能够形成稳定的发夹结构。BGMV DNA中的所有潜在编码区在TGMV和CLV DNA中都有对应区域，这表明基因组组织总体相似，但BGMV DNA中的两个潜在编码区在TGMV DNA中没有对应区域。最保守的开放阅读框BGMV 1R1、TGMV 1R1和CLV 1R1，分别是BGMV、TGMV和CLV外壳蛋白的推定基因。BGMV 1R1与TGMV和CLV的同源性分别为91.9%和71.6%。开放阅读框（BGMV 1L1；CLV 1L1；TGMV 1L1）以及两个较小的重叠开放阅读框（BGMV 1L2、1L3；TGMV 1L2、1L3；CLV 1L5、1L3）在这三种病毒中是保守的。BGMV 2R1和BGMV 2L1与TGMV的同源性较高，但与CLV的2R1和2L1同源性较低。从这项研究中我们得出结论，BGMV与TGMV的关系比与CLV的关系更密切。
