John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K..
Plant Mol Biol. 1985 Mar;5(2):125-31. doi: 10.1007/BF00020095.
The amino acid sequences of the putative polypeptides of maize streak virus (MSV) have been systematically compared with those of cassava latent virus (CLV) and tomato golden mosaic virus (TGMV) using the programme DIAGON (8).Conserved sequences have been detected between peptides encoded by the complementary (-) sense of MSV and those of CLV and TGMV, viz; the 40 200 Mr polypeptide of CLV-1 (3) and the 40 285 Mr polypeptide of TGMV-A (4) show extensive homologies with the 17 768 Mr and 31 388 Mr polypeptides of MSV (6).Distant and variable homologies have been detected between the putative coat protein of MSV when compared with those of CLV and TGMV. No other relationships between the potential gene products of MSV and those of CLV and TGMV have been detected.The extensive homologies detected between the complementary sense encoded peptides suggest that they are derived from functional genes, and that the directly conserved sequences may contain amino acids essential to the function of these proteins. The less extensive homologies among the putative coat proteins are considered in relation to their possible structures and functions.
使用 DIAGON 程序(8),系统地比较了玉米线条病毒(MSV)假定多肽的氨基酸序列与木薯潜隐病毒(CLV)和番茄金黄镶嵌病毒(TGMV)的氨基酸序列。在 MSV 的互补(-)链编码的肽与 CLV 和 TGMV 的相应肽之间检测到保守序列,即 CLV-1 的 40200 Mr 多肽(3)和 TGMV-A 的 40285 Mr 多肽(4)与 MSV 的 17768 Mr 和 31388 Mr 多肽(6)有广泛的同源性。在 MSV 的假定外壳蛋白与其 CLV 和 TGMV 的相应蛋白进行比较时,检测到了遥远和可变的同源性。在 MSV 的潜在基因产物与 CLV 和 TGMV 的相应基因产物之间没有检测到其他关系。在互补链编码的肽之间检测到的广泛同源性表明它们是由功能基因衍生而来的,并且直接保守序列可能包含这些蛋白质功能所必需的氨基酸。在假定的外壳蛋白中,同源性较少,这与它们可能的结构和功能有关。