Faradzhev A N
Vopr Med Khim. 1988 Nov-Dec;34(6):32-6.
Activities of GABA-transaminase (GABA-TA) and glutamate decarboxylase (GDC) were estimated in mitochondria of limbic cortex (L1 and L2 areas), hypothalamus, hippocampus, amygdala and midbrain reticular formation (RF) of 3 months and 1 year old dogs during 5, 12 and 20 days of starvation. Total activity of GABA-TA and GDC was distinctly decreased in mitochondria of limbic cortex, hippocampus, hypothalamus and amygdala. At the same time, after 5 days starvation activity of GDC, in presence of 0.1 mM PALP or without it was increased in RF mitochondria of 3 months old dogs, while GABA-TA was activated in the mitochondria of 1 year old dogs. The rate of GDC activation by PALP was dissimilar in certain structures of brain limbic areas during various periods of starvation in 3 months old dogs, which occurred due to difference of the GDC mitochondrial forms in synaptic structures of the dog brain limbic system. Activity of GABA-TA in 3 months and 1 year old dogs as well as activity of GDC, in presence of PALP or without it, in 3 months old dogs were distinctly decreased in mitochondria of all the dog brain limbic structures studied after long-term deprivation within 12 and 20 days. The decrease in activity of GABA-TA and GDC was related to duration of starvation as well as to morpho-functional alterations and adaptation of the brain structures studied during postnatal ontogenesis.
在3个月和1岁犬类的饥饿5天、12天和20天期间，对其边缘皮质（L1和L2区）、下丘脑、海马体、杏仁核和中脑网状结构（RF）的线粒体中γ-氨基丁酸转氨酶（GABA-TA）和谷氨酸脱羧酶（GDC）的活性进行了评估。边缘皮质、海马体、下丘脑和杏仁核线粒体中GABA-TA和GDC的总活性明显降低。与此同时，饥饿5天后，在0.1 mM磷酸吡哆醛存在或不存在的情况下，3个月龄犬RF线粒体中GDC的活性增加，而1岁犬线粒体中的GABA-TA被激活。在3个月龄犬饥饿的不同时期，边缘脑区某些结构中磷酸吡哆醛对GDC的激活率不同，这是由于犬脑边缘系统突触结构中GDC线粒体形式的差异所致。在长期剥夺12天和20天后，所研究的所有犬脑边缘结构的线粒体中，3个月龄和1岁犬的GABA-TA活性以及3个月龄犬在有或无磷酸吡哆醛情况下的GDC活性均明显降低。GABA-TA和GDC活性的降低与饥饿持续时间以及出生后个体发育过程中所研究脑结构的形态功能改变和适应性有关。
