Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 611731, China.
West China School of Public Health and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, 610041, China.
Anal Sci. 2020 Oct 10;36(10):1191-1195. doi: 10.2116/analsci.20P038. Epub 2020 May 8.
A urinary organic acids profile can be utilized as an effective screening tool for analyzing abnormality of nutrient metabolism. By using these metabolic markers in conjunction with one another, it helps in understanding how individual nutrient metabolism is executed and to determine where there may be imbalances in the metabolic cycle. In this study, we developed a rapid quantification method of 20 urinary organic acids by HPLC-mass spectrometry. A pre-analytical process of organic acid extraction from a urine sample is crucial in this methodology. The process was accomplished by liquid-liquid extraction followed by strong anion exchange. Compared with previous methods, this method greatly reduces the analysis time and allows for the simultaneous quantification of 20 organic acids within 10 min for the first time. This methodology enabled us to analyze urine samples collected from 34 Chinese children. The abnormalities of some urinary organic acids were found in this group, which revealed evidence of functional inadequacy of specific nutrients. The preliminary data in this study confirmed the suitability of the method for rapid and accurate quantification of the target organic acids in urine samples.
尿有机酸谱可作为一种有效的筛选工具，用于分析营养代谢异常。通过共同使用这些代谢标志物，有助于了解个体营养代谢的执行情况，并确定代谢循环中可能存在的不平衡。在这项研究中，我们开发了一种 HPLC-质谱法快速定量 20 种尿有机酸的方法。在该方法中，从尿液样本中提取有机酸的前分析过程至关重要。该过程通过液-液萃取和强阴离子交换完成。与以前的方法相比，该方法大大缩短了分析时间，并首次实现了 10 分钟内同时定量 20 种有机酸。该方法使我们能够分析来自 34 名中国儿童的尿液样本。在该组中发现了一些尿有机酸的异常，这表明特定营养素的功能不足。本研究的初步数据证实了该方法适用于快速准确地定量尿液样本中的目标有机酸。
