Bai Hualong, Wang Zhiwei, Li Mingxing, Sun Peng, Wang Wang, Liu Weizhen, Wei Shunbo, Wang Zhiju, Xing Ying, Dardik Alan
Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China.
Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Henan, China.
Vascular. 2020 Oct;28(5):664-672. doi: 10.1177/1708538120923191. Epub 2020 May 9.
The high rate of clinical failure of prosthetic arteriovenous grafts continues to suggest the need for novel tissue-engineered vascular grafts. We tested the hypothesis that the decellularized rat jugular vein could be successfully used as a conduit and that it would support reendothelialization as well as adaptation to the arterial environment.
Autologous (control) or heterologous decellularized jugular vein (1 cm length, 1 mm diameter) was sewn between the inferior vena cava and aorta as an arteriovenous graft in Wistar rats. Rats were sacrificed on postoperative day 21 for examination.
All rats survived, and grafts had 100% patency in both the control and decellularized groups. Both control and decellularized jugular vein grafts showed similar rates of reendothelialization, smooth muscle cell deposition, macrophage infiltration, and cell turnover. The outflow veins distal to the grafts showed similar adaptation to the arteriovenous flow. Both CD34, CD90 and nestin positive cells, as well as M1-type and M2-type macrophages accumulated around the graft.
This model shows that decellularized vein can be successfully used as an arteriovenous graft between the rat aorta and the inferior vena cava. Several types of cells, including progenitor cells and macrophages, are present in the host response to these grafts in this model. This model can be used to test the application of arteriovenous grafts before conducting large animal experiments.
人工动静脉移植物的临床失败率居高不下，这持续表明需要新型组织工程血管移植物。我们检验了以下假设：脱细胞大鼠颈静脉可成功用作导管，并且它将支持再内皮化以及适应动脉环境。
将自体（对照）或异种脱细胞颈静脉（长度1厘米，直径1毫米）作为动静脉移植物缝合在Wistar大鼠的下腔静脉和主动脉之间。术后第21天处死大鼠进行检查。
所有大鼠均存活，对照组和脱细胞组的移植物通畅率均为100%。对照和脱细胞颈静脉移植物的再内皮化、平滑肌细胞沉积、巨噬细胞浸润和细胞更新率相似。移植物远端的流出静脉对动静脉血流的适应情况相似。CD34、CD90和巢蛋白阳性细胞以及M1型和M2型巨噬细胞均在移植物周围聚集。
该模型表明，脱细胞静脉可成功用作大鼠主动脉和下腔静脉之间的动静脉移植物。在该模型中，宿主对这些移植物的反应中存在几种类型的细胞，包括祖细胞和巨噬细胞。该模型可用于在进行大型动物实验之前测试动静脉移植物的应用。
