Song Yue, Song Wei, Li Zhaoming, Song Wenting, Wen Yibo, Li Jiwei, Xia Qingxin, Zhang Mingzhi
Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
The Academy of Medical Science of Zhengzhou University, Zhengzhou, China.
Front Oncol. 2020 Apr 23;10:488. doi: 10.3389/fonc.2020.00488. eCollection 2020.
T-lymphoblastic lymphoma (T-LBL) is a rare hematological malignancy with highly aggressive, unique clinical manifestations, and poor prognosis. Cell division cycle 27 (CDC27) was previously reported to be a significant subunit of the anaphase-promoting complex/cyclosome. However, the specific functions and relevant mechanisms of CDC27 in T-LBL remain unknown. Through immunohistochemistry staining, we identified that CDC27 was overexpressed in T-LBL tissues and related to tumor progression and poor survival. Functional experiments demonstrated that CDC27 promoted proliferation and . Further experiment suggested the role of CDC27 in facilitating G1/S transition and promoting the expression of Cyclin D1 and CDK4. Then the effect of CDC27 in inhibiting apoptosis was also identified. Furthermore, we found a positive correlation between the expression of CDC27 and Programmed death ligand-1 (PD-L1) by immunohistochemistry staining. The interaction between CDC27 and PD-L1 was also proved by western blot, luciferase gene reporter assay and immunofluorescence. Taken together, our results showed that CDC27 contributes to T-LBL progression and there is a positive correlation between PD-L1 and CDC27, which offers novel perspectives for future studies on targeting CDC27 in T-LBL.
T淋巴细胞母细胞淋巴瘤（T-LBL）是一种罕见的血液系统恶性肿瘤，具有高度侵袭性、独特的临床表现且预后较差。细胞分裂周期27（CDC27）先前被报道为后期促进复合体/细胞周期体的一个重要亚基。然而，CDC27在T-LBL中的具体功能及相关机制仍不清楚。通过免疫组织化学染色，我们发现CDC27在T-LBL组织中过表达，且与肿瘤进展及不良生存相关。功能实验表明，CDC27促进增殖以及……进一步实验提示CDC27在促进G1/S期转换及促进细胞周期蛋白D1和细胞周期蛋白依赖性激酶4表达方面的作用。随后还确定了CDC27在抑制细胞凋亡方面的作用。此外，通过免疫组织化学染色我们发现CDC27与程序性死亡配体-1（PD-L1）的表达呈正相关。蛋白质免疫印迹法、荧光素酶基因报告基因检测及免疫荧光法也证实了CDC27与PD-L1之间的相互作用。综上所述，我们的结果表明CDC27促进T-LBL进展，且PD-L1与CDC27呈正相关，这为未来针对T-LBL中CDC27的研究提供了新的视角。
