Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
Nutrients. 2020 May 7;12(5):1334. doi: 10.3390/nu12051334.
During pregnancy, most women are exposed to caffeine, which is a widely consumed psychoactive substance. However, the consequences of maternal caffeine intake on the child remain largely unknown. Here, we investigated the intergenerational effects of maternal caffeine intake on offspring in a model. We treated a young mother (P0) with 10 mM of caffeine equivalent to 2-5 cans of commercial energy drinks and examined its reproduction and growth rate from P0 to F2 generation. The fertility decreased and embryonic lethality increased by defective oocytes and eggshell integrity in caffeine-ingested mothers, and F1 larval development severely retarded. These results were due to decreased production of vitellogenin protein (yolk) in caffeine-ingested mothers. Furthermore, effects of RNA interference of vitellogenin () genes, to , in P0 mothers can mimic those by caffeine-ingested mothers. In addition, RNA interference (RNAi) depletion of (human Meis homeobox), a transcriptional activator for genes, also showed similar effects induced by caffeine intake. Taken together, maternal caffeine intake reduced yolk production mediated by the UNC-62 transcription factor, thereby disrupting oocyte and eggshell integrity and retarding larval development. Our study suggests the clinical significance of caffeine intake for prospective mothers.
在怀孕期间，大多数女性都接触过咖啡因，这是一种广泛消费的精神活性物质。然而，母亲摄入咖啡因对孩子的影响在很大程度上仍然未知。在这里，我们在一个 模型中研究了母体咖啡因摄入对后代的代际影响。我们用相当于 2-5 罐商业能量饮料的 10mM 咖啡因处理一位年轻的母亲 (P0)，并从 P0 到 F2 代检查其繁殖和生长速度。咖啡因摄入母亲的卵母细胞和蛋壳完整性缺陷导致生育力下降和胚胎致死率增加，F1 幼虫发育严重延迟。这些结果是由于咖啡因摄入母亲产生的卵黄蛋白原（蛋黄）蛋白减少所致。此外，用 RNA 干扰 P0 母亲的 vitellogenin () 基因，从 到 ，可以模拟咖啡因摄入母亲的效果。此外，UNC-62 转录因子的转录激活剂 vitellogenin () 基因的 RNA 干扰 (RNAi) 耗竭也显示出类似的咖啡因摄入诱导的效果。总之，母体咖啡因摄入通过 UNC-62 转录因子减少蛋黄的产生，从而破坏卵母细胞和蛋壳的完整性并延迟幼虫的发育。我们的研究提示临床医生母亲摄入咖啡因的重要性。
