咖啡因及相关甲基黄嘌呤的促氧化和寿命延长作用于…… （文本不完整，翻译至此）

Pro-oxidant and lifespan extension effects of caffeine and related methylxanthines in .

作者信息

Li Hanmei, Roxo Mariana, Cheng Xinlai, Zhang Shaoxiong, Cheng Haoran, Wink Michael

机构信息

Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany.

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

Food Chem X. 2019 Jan 19;1:100005. doi: 10.1016/j.fochx.2019.100005. eCollection 2019 Mar 30.

Caffeine and related purine alkaloids are common ingredients of many stimulating drinks. Studies have shown that lower concentrations of caffeine have a protective role in aging-related disorders. However, the associated mode of action of caffeine and its related methylxanthines is still not clear. In this study, we demonstrated that caffeine and theophylline promote longevity in . Lifespan studies with the wild type, DAF-16 and SKN-1 mutant strains indicated that the methylxanthines-mediated lifespan extension in was independent of DAF-16/FOXO and SKN-1. All the tested methylxanthines could protect against acute oxidative stress. At early stages of life, an increase of ROS (reactive oxygen species) induced the translocation of DAF-16 and SKN-1, resulting in upregulation of several antioxidant genes, for example, ::GFP, ::GFP, ::GFP; and downregulation of ::GFP. RT-PCR corroborates the upregulation of and genes. The expression of DAF-16 decreased although its nuclear translocation was induced.

咖啡因及相关嘌呤生物碱是许多刺激性饮品的常见成分。研究表明，较低浓度的咖啡因在与衰老相关的病症中具有保护作用。然而，咖啡因及其相关甲基黄嘌呤的相关作用模式仍不清楚。在本研究中，我们证明了咖啡因和茶碱可延长秀丽隐杆线虫的寿命。对野生型、DAF-16和SKN-1突变株的寿命研究表明，甲基黄嘌呤介导的秀丽隐杆线虫寿命延长与DAF-16/FOXO和SKN-1无关。所有测试的甲基黄嘌呤都能保护秀丽隐杆线虫免受急性氧化应激。在生命早期，活性氧（ROS）的增加诱导了DAF-16和SKN-1的易位，导致几个抗氧化基因的上调，例如，::GFP、::GFP、::GFP；以及::GFP的下调。RT-PCR证实了和基因的上调。尽管诱导了DAF-16的核易位，但其表达却下降了。