Institute of Biomaterials and Biomedical Engineering, Faculty of Applied Science and Engineering, University of Toronto, Toronto, ON, Canada.
Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada.
Commun Biol. 2020 May 11;3(1):229. doi: 10.1038/s42003-020-0946-9.
Primary adult cardiomyocyte (aCM) represent the mature form of myocytes found in the adult heart. However, culture of aCMs in particular is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca cycling to enable physiologically relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance. Together, these refinements to aCM culture present a toolbox with which to advance our understanding of myocardial physiology.
原代成年心肌细胞（aCM）代表成熟的心肌细胞，存在于成年心脏中。然而，aCM 的培养尤其具有挑战性，因为其存活率低，表型丧失，导致体外实验无法进行。在这里，我们建立了增强 aCM 存活并维持肌节结构和 Ca 循环的方法，使生理相关的收缩力测量成为可能。我们还证明了遗传和小分子操作，可以探测肌细胞功能表现的机制。总之，这些对 aCM 培养的改进提供了一个工具包，可以帮助我们深入了解心肌生理学。
