Cancer Research UK, University of Cambridge, Cambridge, UK.
Abcam plc, Discovery Drive, Cambridge Biomedical Campus, Cambridge, UK.
Methods Mol Biol. 2020;2148:3-17. doi: 10.1007/978-1-0716-0623-0_1.
As RNA in situ hybridization (ISH) moves into the mainstream lab and increasingly into clinical adoption and additional multiplexing techniques are developed to enable further RNA ISH identification, a set of guidelines on the validation of ISH is required. These guidelines include choice of methods, appropriate controls, and protocol optimization as well as a central core message of understanding the target, understanding the ISH technique, and using the most appropriate controlling mechanisms to enable reproducible and trustworthy data to be obtained.
随着 RNA 原位杂交（ISH）技术逐渐进入主流实验室，并越来越多地被临床采用，以及更多的多重技术被开发以实现进一步的 RNA ISH 鉴定，因此需要制定一套关于 ISH 验证的指南。这些指南包括方法选择、适当的对照以及方案优化，以及一个核心信息，即了解靶标、了解 ISH 技术，并使用最合适的控制机制，以获得可重复和可靠的数据。
