Institute of Molecular Medicine, Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Angew Chem Int Ed Engl. 2020 Aug 10;59(33):14115-14119. doi: 10.1002/anie.202005974. Epub 2020 Jun 4.
Microfluidic chips with nano-scale structures have shown great potential, but the fabrication and cost issues restrict their application. Herein, we propose a conceptually new "DNA nanolithography in a microfluidic chip" by using sub-10 nm three-dimensional DNA structures (TDNs) as frameworks with a pendant aptamer at the top vertex (ApTDN-Chip). The nano-scale framework ensures that the aptamer is in a highly ordered upright orientation, avoiding the undesired orientation or crowding effects caused by conventional microfluidic interface fabrication processes. Compared with a monovalent aptamer modified chip, the capture efficiency of ApTDN-Chip was enhanced nearly 60 % due to the highly precise dimension and rigid framework of TDNs. In addition, the scaffolds make DNase I more accessible to the aptamer with up to 83 % release efficiency and 91 % cell viability, which is fully compatible with downstream molecular analysis. Overall, this strategy provides a novel perspective on engineering nano-scaffolds to achieve a more ordered nano-topography of microfluidic chips.
具有纳米结构的微流控芯片具有巨大的潜力，但制造和成本问题限制了它们的应用。在这里，我们提出了一种新概念的“微流控芯片中的 DNA 纳米光刻”，使用亚 10nm 的三维 DNA 结构（TDN）作为框架，并在顶部顶点带有一个悬挂适体（ApTDN-Chip）。纳米级框架确保适体处于高度有序的直立状态，避免了传统微流控界面制造工艺引起的不适宜取向或拥挤效应。与单价适体修饰的芯片相比，由于 TDN 的高度精确尺寸和刚性框架，ApTDN-Chip 的捕获效率提高了近 60%。此外，支架使 DNase I 更容易接近适体，释放效率高达 83%，细胞活力高达 91%，完全兼容下游分子分析。总的来说，该策略为工程纳米支架提供了一个新的视角，以实现微流控芯片更有序的纳米形貌。
