1Francis I. Proctor Foundation, University of California, San Francisco, San Francisco, California.
2Division of Epidemiology, School of Public Health, University of California, Berkeley, Berkeley, California.
Am J Trop Med Hyg. 2020 Jul;103(1):234-237. doi: 10.4269/ajtmh.19-0695. Epub 2020 May 7.
Nucleic acid amplification tests are increasingly used to detect ocular chlamydia infection in trachoma research and programs. To evaluate the reliability of detection by the Abbott RealTime CT/NG assay (Abbott Molecular, Inc., Des Plaines, IL) on the m2000 platform, three conjunctival samples were collected from each of 200 children aged 0-9 years in Ethiopia: two from the right eye and one from the left eye. Four aliquots were processed for each child: two from the first right eye sample, one from the second right eye sample, and one from the left eye sample. Sixty-nine swabs were processed in a U.S. laboratory and 131 in an Ethiopian laboratory. Intra-class correlation coefficients (ICCs) were high when comparing two aliquots from the same swab (ICC ranged from 0.96 to 0.99), two separate swabs from the right eye (0.89-0.91), and one right and one left eye swab (0.87-0.89), indicating reliable chlamydial load assessment across different samples and laboratory settings.
核酸扩增检测越来越多地用于在沙眼研究和项目中检测眼部衣原体感染。为了评估 Abbott RealTime CT/NG 检测试剂盒（ Abbott Molecular，Inc.，Des Plaines，IL）在 m2000 平台上的检测可靠性，从埃塞俄比亚 200 名 0-9 岁儿童的每只眼中采集了三个结膜样本：右眼两个，左眼一个。每个儿童处理了四个等分试样：两个来自第一个右眼样本，一个来自第二个右眼样本，一个来自左眼样本。69 个拭子在美国实验室处理，131 个在埃塞俄比亚实验室处理。当比较来自同一拭子的两个等分试样（ICC 范围为 0.96 至 0.99）、右眼的两个单独拭子（0.89-0.91）和一个右眼和一个左眼拭子时，内类相关系数（ICC）很高（0.87-0.89），表明在不同样本和实验室环境中具有可靠的衣原体负荷评估能力。
