Turek Ilona, Irving Helen, Gehring Chris
Biomolecular Laboratory, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Department of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Science, La Trobe University, Bendigo, Australia.
Data Brief. 2020 Apr 22;30:105606. doi: 10.1016/j.dib.2020.105606. eCollection 2020 Jun.
Interactors of the plant natriuretic peptide present in , termed AtPNP-A, were affinity-based isolated from (Col-0) leaf mesophyll cell protoplasts by incubating the protoplasts with biologically active biotinylated peptide corresponding to amino acid sequence of the active site of AtPNP-A (pAtPNP-A), either in the presence or absence of a cross-linking agent, 3,3'-dithiobis(sulfosuccinimidyl propionate) (DTSSP), or with equimolar amount of biotin with DTSSP (negative control). Upon biotin/streptavidin-based isolation of proteins bound to the pAtPNP-A or biotin, the proteins were separated by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE), digested with trypsin and subjected to identification with liquid chromatography tandem mass spectrometry (LC-MS/MS). Label-free quantification of identified proteins allowed identification of binding partners of AtPNP-A, paving the way for pinpointing novel signal transduction pathways AtPNP-A is involved in. The raw and processed LC-MS/MS data reported in this article have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD017925.
存在于拟南芥中的植物利尿钠肽（称为AtPNP-A）的相互作用分子，通过将（Col-0）叶片叶肉细胞原生质体与对应于AtPNP-A活性位点氨基酸序列的生物活性生物素化肽（pAtPNP-A）孵育，在存在或不存在交联剂3,3'-二硫代双（磺基琥珀酰亚胺丙酸酯）（DTSSP）的情况下，或与等摩尔量的生物素和DTSSP（阴性对照）一起，基于亲和力从原生质体中分离出来。在基于生物素/链霉亲和素分离与pAtPNP-A或生物素结合的蛋白质后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离蛋白质，用胰蛋白酶消化并通过液相色谱串联质谱（LC-MS/MS）进行鉴定。对鉴定出的蛋白质进行无标记定量，从而鉴定出AtPNP-A的结合伴侣，为确定AtPNP-A参与的新信号转导途径铺平了道路。本文报道的原始和处理后的LC-MS/MS数据已以数据集标识符PXD017925存入蛋白质组交换联盟。
