Filatova A Yu, Potekhina A V, Pylaeva E A, Osokina A K, Ruleva N Yu, Pogorelova O A, Tripoten M I, Noeva E A, Balakhonova T V, Masenko V P, Arefieva T I
Laboratory of Cell Immunology of Institute of Experimental Cardiology of FSBO, National Medical Research Center of Cardiology of Russian Ministry of Health, Russia.
Department of Pulmonary Hypertension and Heart Diseases of Institute of Clinical Cardiology of FSBO, National Medical Research Center of Cardiology of Russian Ministry of Health, Russia.
Heliyon. 2020 May 5;6(5):e03856. doi: 10.1016/j.heliyon.2020.e03856. eCollection 2020 May.
Immune and inflammatory reactions contribute to the progression of atherosclerosis. The walls of the different arteries and segments of the arteries have heterogeneous haemodynamic and histological features. We aimed to explore the relationship between the circulating T-cell subsets and the abundance of carotid atherosclerosis in different segments of carotid arteries.
70 patients underwent ultrasound duplex scanning to determine the degree of stenosis of the common carotid artery (CCA), the CCA bifurcation or the internal carotid artery (ICA). The blood frequencies of T-, B-, NK-cells, regulatory T cells (Treg), activated T-helpers (Th), IL10-producing Th, Th1 and Th17, as well as blood levels of hsCRP, sCD25, IL10 and IL17a were assessed.
The frequencies of Th17 were increased in patients with ICA stenosis >35% and >50% vs. patients with ICA stenosis <35%. Th17 blood level ≥0.55 % of lymphocytes was associated with more severe stenosis of ICA (OR 4.3 (1.0-17.6), p < 0.05 for ICA stenosis of 35-50% and 6.8 (1.3-35.0), p < 0.05 for ICA stenosis >50%). BMI positively correlated with the CCA bifurcation stenosis degree (r = 0.33, p < 0.05).
The severity of ICA stenosis can be associated with the circulating Th17 level.
免疫和炎症反应促进动脉粥样硬化的进展。不同动脉及其各节段的血管壁具有异质性的血流动力学和组织学特征。我们旨在探讨循环T细胞亚群与颈动脉不同节段颈动脉粥样硬化程度之间的关系。
70例患者接受了超声双功扫描，以确定颈总动脉（CCA）、CCA分叉处或颈内动脉（ICA）的狭窄程度。评估了T细胞、B细胞、NK细胞、调节性T细胞（Treg）、活化辅助性T细胞（Th）、产生IL-10的Th、Th1和Th17的血液频率，以及hsCRP、sCD25、IL-10和IL-17a的血液水平。
与ICA狭窄<35%的患者相比，ICA狭窄>35%和>50%的患者中Th17频率增加。Th17血液水平≥淋巴细胞的0.55%与ICA更严重的狭窄相关（ICA狭窄35%-50%时，比值比为4.3（1.0-17.6），p<0.05；ICA狭窄>50%时，比值比为6.8（1.3-35.0），p<0.05）。BMI与CCA分叉处狭窄程度呈正相关（r=0.33，p<0.05）。
ICA狭窄的严重程度可能与循环Th17水平有关。
