Filatova A Yu, Pylaeva E A, Potekhina A V, Osokina A K, Pogorelova O A, Tripoten M I, Balakhonova T V, Provatorov S I, Noeva E A, Klesareva E A, Afanasieva O I, Arefieva T I
Russian Cardiology Research and Production Complex, Moscow, Russia.
Kardiologiia. 2017 Apr;57(4):64-71.
to assess prognostic significance of blood content of regulatory and effector T-lymphocytes for progression of atherosclerosis (AS) of carotid arteries.
We enrolled in this study 33 men with various severity of carotid AS. Carotid artery duplex scan was done at admission and in 1 year after enrollment. AS progression was defined as appearance of novel stenosis in common or internal carotid artery or more or equal 5% increase of preexisting stenosis. Peripheral blood lymphocyte phenotyping was performed by direct immunofluorescence and flow cytometry at the enrollment. T-helpers (Th) 1 were identified as CD4+IFNgamma+ cells, Th2 - CD4+IL4+, activated T-cells (T-act) - D4+CD25lowCD127high, regulatory T-cells (T-reg) - D4+CD25highCD127 low and CD4+FoxP3+, Th17 - CD4+IL17a+ cells.
Progression of carotid AS was observed in 18 patients. Basal values of Th17 were higher while ratio T-reg/Th17 was lower in patients with compared with those without AS progression. ROC-analysis showed high sensitivity and specificity of blood levels of Th17, T-act and T-reg/Th17 ratio for carotid AS progression during one year in patients with low density lipoprotein cholesterol (LDLCH) level below 3.5 mmol/l.
The imbalance between circulating levels of regulatory T-cells and T-helpers 17 with the prevalence of proinflammatory T-helpers 17 may reflect a predisposition for carotid AS progression, what also refers to patients with relatively low LDLCH.
评估调节性和效应性T淋巴细胞的血液含量对颈动脉动脉粥样硬化（AS）进展的预后意义。
我们招募了33名患有不同严重程度颈动脉AS的男性。入院时及入组后1年进行颈动脉双功扫描。AS进展定义为颈总动脉或颈内动脉出现新的狭窄或原有狭窄增加5%及以上。入组时通过直接免疫荧光和流式细胞术进行外周血淋巴细胞表型分析。辅助性T细胞（Th）1被鉴定为CD4+IFNγ+细胞，Th2为CD4+IL4+，活化T细胞（T-act）为D4+CD25lowCD127high，调节性T细胞（T-reg）为D4+CD25highCD127low和CD4+FoxP3+，Th17为CD4+IL17a+细胞。
18例患者观察到颈动脉AS进展。与无AS进展的患者相比，AS进展患者的Th17基础值较高，而T-reg/Th17比值较低。ROC分析显示，在低密度脂蛋白胆固醇（LDLCH）水平低于3.5 mmol/l的患者中，Th17、T-act的血液水平及T-reg/Th17比值对颈动脉AS在1年内进展具有较高的敏感性和特异性。
调节性T细胞和辅助性T细胞17循环水平失衡，促炎性辅助性T细胞17占优势，可能反映了颈动脉AS进展的易感性，这也适用于LDLCH相对较低的患者。
