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来自顶芽的植株再生。

Plant regeneration in from apical bud.

作者信息

Zhang Huijuan, Qin Shifei, Cao Hui, Zhang Qingwei, Luo Keming

机构信息

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China.

Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China.

出版信息

Heliyon. 2020 May 3;6(5):e03884. doi: 10.1016/j.heliyon.2020.e03884. eCollection 2020 May.

Abstract

is a woody plant species with important economic value, its seeds could be used to produce biodiesel and the oil extracted from them serve many kinds of industrial applications. In this study, we established a regeneration system of for the first time using apical buds as explants on the Murashige and Skoog (MS) medium supplemented with the plant growth regulators, 6-benzylaminopurine (6-BA) and 3-indolebutyric acid (IBA). The optimal condition for adventitious bud induction was MS medium containing 1.5 mg/L 6-BA and 0.01 mg/L IBA, which produced a satisfactory induction efficiency. In subculture, the MS medium containing 1.0 mg/L 6-BA and 0.01 mg/L IBA was the optimum selection when considering both proliferation rate and tissue growth status. The adventitious shoots inoculated on 1/2 MS medium containing 0.5 mg/L IBA could produce strong roots and 96.4% of the regenerated plants survived after transplanting.

摘要

是一种具有重要经济价值的木本植物物种,其种子可用于生产生物柴油,从中提取的油有多种工业用途。在本研究中,我们首次以顶芽为外植体,在添加了植物生长调节剂6-苄基腺嘌呤(6-BA)和3-吲哚丁酸(IBA)的Murashige和Skoog(MS)培养基上建立了其再生体系。不定芽诱导的最佳条件是含有1.5 mg/L 6-BA和0.01 mg/L IBA的MS培养基,其诱导效率令人满意。在继代培养中,考虑到增殖率和组织生长状态,含有1.0 mg/L 6-BA和0.01 mg/L IBA的MS培养基是最佳选择。接种在含有0.5 mg/L IBA的1/2 MS培养基上的不定芽能产生强壮的根,96.4%的再生植株移栽后存活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9701/7205760/be48a4ac2b25/gr1.jpg

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