Identification of Resistance Genes to in Domestic Soybean Cultivars from China Using Particle Bombardment.

Phytophthora root and stem rot caused by is a destructive disease that afflicts soybean plants throughout the world. The use of resistant soybean cultivars is the primary means of managing this disease, as well as the most effective and economical approach. There are abundant soybean germplasm resources in China that could be deployed for breeding programs; however, the resistance genes ( genes) in most cultivars are unknown, leading to uncertainty concerning which are resistant cultivars for use. The resistance genes , , and prevent root and stem rot caused by most . isolates within a Chinese field population. This study identified three genes in Chinese domestic soybean cultivars using three related avirulence genes by particle bombardment. The complex genetic diversity of soybean cultivars and . strains has made it difficult to define single genes without molecular involvement. Gene cobombardment is a method for identifying genes quickly and specifically. We showed that cultivars Dongnong 60 and Henong 72 contained , while Hedou 19, Henong 76, 75-3, Wandou 21020, Zheng 196, Wandou 28, Heinong 71, and Wandou 29 all contained . The cultivars Jidou 12, Henong 72, Heinong 71, and Wandou 29 contained . The cultivar Henong 72 contained both and , while Wandou 29 and Heinong 71 contained both and . We then evaluated the phenotype of 11 domestic soybean cultivars reacting to . using the isolates P6497 and Ps1. The 11 domestic cultivars were all resistant to P6497 and Ps1. This research provides source materials and parent plant strains containing , , and for soybean breeding programs.