Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
Anat Rec (Hoboken). 2021 Feb;304(2):393-399. doi: 10.1002/ar.24418. Epub 2020 Jun 23.
The present study aims to give detailed histomorphological features of the hippocampus of adult male New Zealand rabbits. Both histological and histochemical specimens were prepared to be examined microscopically by using a light microscope. The hippocampus appeared as C-shaped hippocampal proper, dentate gyrus, and subiculum. The hippocampal proper subdivided along its length according to the density and size of its major constituent pyramidal cells into four distinct regions named Cornu Ammonis (CA1, CA2, CA3, and CA4). With the histochemical preparations, each of these regions consisted of five layers, stratum alveolus, stratum oriens, stratum pyramidale, stratum radiatum, and stratum lacunosum-moleculare. The stratum pyramidale constituted the middle dark zone and contained the principal excitatory neurons and a few interneurons. Histochemically, the pyramidal neurons along all regions of the CA reacted positively to Grimelius silver impregnation, lead hematoxylin, Gomori's aldehyde fuchsin, aldehyde thionine, Gomori's chrome alum hematoxylin, and performic acid alcian blue stains. Immunohistochemically, the pyramidal neurons reacted positively to anti-NSE antibodies. The dentate gyrus was formed of three distinct layers. The subiculum was formed of proper subiculum, presubiculum, and parasubiculum.
本研究旨在详细描述成年雄性新西兰兔海马的组织形态学特征。通过使用光学显微镜，对组织学和组织化学标本进行了检查。海马呈现出 C 形的海马体、齿状回和下托。海马体根据其主要组成的锥体细胞的密度和大小沿其长度分为四个不同的区域，分别命名为齿状回（CA1、CA2、CA3 和 CA4）。通过组织化学制备，这些区域中的每一个都由五个层组成，分别是齿状回颗粒层、始层、锥体层、放射层和腔隙分子层。锥体层构成了中间暗带，包含主要的兴奋性神经元和少数中间神经元。组织化学染色显示，CA 各区域的锥体神经元对格里姆利银浸渍、铅苏木精、戈尔莫利醛品红、醛硫堇、戈尔莫利铬明矾苏木精和过甲酸阿尔辛蓝染色呈阳性反应。免疫组织化学染色显示，锥体神经元对 NSE 抗体呈阳性反应。齿状回由三个不同的层组成。下托由固有下托、前下托和副下托组成。
