Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan, 44610, South Korea.
Zoological Survey of India, Prani Vigyan Bhawan, M-Block, New Alipore, Kolkata, 700053, India.
J Eukaryot Microbiol. 2020 Sep;67(5):541-554. doi: 10.1111/jeu.12801. Epub 2020 May 31.
The morphology and morphogenesis of Rigidohymena inquieta (Stokes, 1887) Berger, 2011, isolated from a lawn soil in the campus of the University of Ulsan, Korea, was studied, using live observation and protargol impregnation. The molecular phylogeny was studied based on the SSU rRNA gene sequences. The morphology of the Korean population of R. inquieta matches the previously known populations; however, the morphogenetic pattern shows differences to the species R. candens in the involvement of cirrus V/3 in the anlagen formation. A novel genus namely Metahymena gen. nov. has been erected for the present species based on the ontogenetic difference, and the new combination Metahymena inquieta gen. nov., comb. nov. is proposed. The morphology, morphogenesis, distribution, and phylogeny of M. inquieta are presented. The morphologic and morphogenetic data corroborate the phylogenetic analyses as M. inquieta clusters among the stylonychid ciliates in a clade distant from Rigidohymena candens.
从韩国蔚山大学大学校园草坪中分离出的 Rigidohymena inquieta（Stokes，1887） Berger，2011，使用活体观察和蛋白银浸渍法对其形态和发生进行了研究。基于 SSU rRNA 基因序列研究了分子系统发育。韩国 Rigidohymena inquieta 种群的形态与先前已知的种群相匹配；然而，发生模式与物种 R. candens 不同，在 cirrus V/3 参与原基形成。基于发生上的差异，为目前的物种建立了一个新属，即 Metahymena gen. nov.，并提出了新的组合 Metahymena inquieta gen. nov.，comb. nov.。介绍了 Metahymena inquieta 的形态、发生、分布和系统发育。形态学和发生学数据支持系统发育分析，因为 M. inquieta 在 Stylonychia 纤毛类群中聚类，与 Rigidohymena candens 相距较远。
