Yang Caiting, Liu An, Xu Yusen, Xu Yuan, Fan Xinpeng, Al-Farraj Saleh A, Ni Bing, Gu Fukang
School of Life Sciences, East China Normal University, Shanghai 200062, China;; Email: unknown.
Laboratory of Protozoology, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, South China Normal University, Guangzhou 510631, China;; Email: unknown.
Zootaxa. 2015 Aug 18;4000(4):451-63. doi: 10.11646/zootaxa.4000.4.4.
The morphology and infraciliature of the four hypotrichous ciliates; Rigidohymena inquieta (Stokes, 1887) Berger, 2011, Pattersoniella vitiphila Foissner, 1987, Notohymena australis Foissner & O' Donoghue, 1990, and Cyrtohymena (Cyrtohymenides) australis (Foissner, 1995) Foissner, 2004, collected from east China, were investigated by using live observation and protargol impregnation method. An improved diagnosis for R. inquieta was supplied based on descriptions of present and previous populations. New morphology and morphogenesis information based on Chinese populations of another three hypotrichids were also supplemented. The Small-subunit rRNA (SSU rRNA) gene sequences of the four species were characterized and their phylogenetic positions were revealed by means of Bayesian inference and Maximum-likelihood analysis. The analyses shows that R. inquieta clusters with other members of the subfamily Stylonychinae, which confirms the monophyly of the subfamily and verified R. inquieta as a separated species from R. candens though it differs from others mainly by body size. C. (C.) australis occupying the basal position of the clade which contains cyrtohymenids and some other groups, declines the idea of separating Cyrtohymena into two subgenus. Notohymena australis and China population of Pattersoniella vitiphila respectively clustering with their congeners correspond well with the systematics revealed by morphological similarities.
利用活体观察和蛋白银染色法，对采自中国东部的四种下毛目纤毛虫，即不安硬膜虫（Rigidohymena inquieta，斯托克斯，1887；伯杰，2011）、嗜葡萄帕特森虫（Pattersoniella vitiphila，福斯纳，1987）、南方诺托膜虫（Notohymena australis，福斯纳和奥多诺霍，1990）以及南方弯膜虫（Cyrtohymena (Cyrtohymenides) australis，福斯纳，1995；福斯纳，2004）的形态和纤毛下器进行了研究。基于对当前和以往种群的描述，提供了对不安硬膜虫的改进诊断。还补充了另外三种下毛目纤毛虫中国种群的新形态和形态发生信息。对这四个物种的小亚基核糖体RNA（SSU rRNA）基因序列进行了特征分析，并通过贝叶斯推断和最大似然分析揭示了它们的系统发育位置。分析表明，不安硬膜虫与毛触亚科的其他成员聚类，这证实了该亚科的单系性，并验证了不安硬膜虫是一个与明亮硬膜虫不同的独立物种，尽管它与其他物种的主要区别在于体型。南方弯膜虫占据包含弯膜虫类和其他一些类群的进化枝的基部位置，这削弱了将弯膜虫分为两个亚属的观点。南方诺托膜虫和嗜葡萄帕特森虫的中国种群分别与其同属聚类，这与形态相似性所揭示的系统分类学结果相符。
