Petersen Andreas Munk, Mirsepasi-Lauridsen Hengameh Chloé, Vester-Andersen Marianne K, Sørensen Nikolaj, Krogfelt Karen Angeliki, Bendtsen Flemming
Gastrounit, Medical Section, Amager-Hvidovre University Hospital, 2650 Copenhagen, Denmark.
Department of Clinical Microbiology, Amager-Hvidovre University Hospital, 2650 Copenhagen, Denmark.
Antibiotics (Basel). 2020 May 8;9(5):237. doi: 10.3390/antibiotics9050237.
Low diversity intestinal dysbiosis has been associated with inflammatory bowel disease, including patients with ulcerative colitis with an ileo-anal pouch anastomosis. Furthermore, specific phylogroups have been linked to inflammatory bowel disease. Our aim was to characterize the differences among microbiota and phylogroups in active and inactive pouchitis. Disease activity was assessed using the modified pouch disease activity index and by fecal calprotectin. Microbiota diversity was assessed by 16S rDNA MiSeq sequencing. phylogroup was determined after triplex PCR. Twenty patients with ulcerative colitis with an ileo-anal pouch anastomosis were included, 10 of whom had active pouchitis. Ileo-anal pouch anastomosis patients had an increased abundance of Proteobacteria colonization compared to patients with ulcerative colitis or Crohn's disease and healthy controls, = 1.4·10. No differences in phylogroup colonization could be determined between cases of active and inactive disease. No significant link was found between α-diversity and pouch inflammation. However, higher levels of Fusobacteria colonization were found in patients with a pouch with a fecal calprotectin level above 500, = 0.02. In conclusion, patients with a pouch had an increased Proteobacteria abundance, but only Fusobacteria abundance was linked to inflammation.
低多样性肠道菌群失调与炎症性肠病有关，包括患有回肠肛管吻合术的溃疡性结肠炎患者。此外，特定的菌系已被证明与炎症性肠病有关。我们的目的是描述活动性和非活动性袋炎患者微生物群和菌系之间的差异。使用改良的袋病活动指数和粪便钙卫蛋白评估疾病活动度。通过16S rDNA MiSeq测序评估微生物群多样性。通过三重PCR确定菌系。纳入了20例患有回肠肛管吻合术的溃疡性结肠炎患者，其中10例患有活动性袋炎。与溃疡性结肠炎、克罗恩病患者及健康对照相比，回肠肛管吻合术患者的变形菌定植丰度增加，P = 1.4·10。在活动性和非活动性疾病病例之间未发现菌系定植存在差异。未发现α多样性与袋炎之间存在显著关联。然而，粪便钙卫蛋白水平高于500的袋炎患者中梭杆菌定植水平更高，P = 0.02。总之，袋炎患者的变形菌丰度增加，但只有梭杆菌丰度与炎症相关。
