State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan 430072, China.
Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06519, USA.
Viruses. 2020 May 8;12(5):519. doi: 10.3390/v12050519.
Mosquito-borne Zika virus (ZIKV) can cause congenital microcephaly and Guillain-Barré syndrome, among other symptoms. Specific treatments and vaccines for ZIKV are not currently available. To further understand the host factors that support ZIKV replication, we used mass spectrometry to characterize mammalian proteins that associate with the ZIKV NS1 protein and identified the TRiC/CCT complex as an interacting partner. Furthermore, the suppression of CCT2, one of the critical components of the TRiC/CCT complex, inhibited ZIKV replication in both mammalian cells and mosquitoes. These results highlight an important role for the TRiC/CCT complex in ZIKV infection, suggesting that the TRiC/CCT complex may be a promising therapeutic target.
蚊媒传播的 Zika 病毒（ZIKV）可引起先天性小头畸形和格林-巴利综合征等症状。目前尚无针对 ZIKV 的特定治疗方法和疫苗。为了进一步了解支持 ZIKV 复制的宿主因素，我们使用质谱法鉴定了与 ZIKV NS1 蛋白结合的哺乳动物蛋白，并鉴定出 TRiC/CCT 复合物是一个相互作用的伙伴。此外，抑制 TRiC/CCT 复合物的关键成分 CCT2 可抑制哺乳动物细胞和蚊子中的 ZIKV 复制。这些结果突出了 TRiC/CCT 复合物在 ZIKV 感染中的重要作用，表明 TRiC/CCT 复合物可能是一个有前途的治疗靶点。
