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用于递送基因药物的光敏聚-L-赖氨酸/肝素聚电解质复合物

Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs.

作者信息

Korzhikov-Vlakh Viktor, Katernuk Iuliia, Pilipenko Iuliia, Lavrentieva Antonina, Guryanov Ivan, Sharoyko Vladimir, Manshina Alina A, Tennikova Tatiana B

机构信息

Institute of Chemistry, Saint Petersburg State University, St. Petersburg, Universitetskii pr. 26, Peterhoff 198504, Russia.

Institute of Technical Chemistry, Leibniz University, Callinstrasse 5, 30167 Hannover, Germany.

出版信息

Polymers (Basel). 2020 May 8;12(5):1077. doi: 10.3390/polym12051077.

DOI:10.3390/polym12051077
PMID:32397208
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7285230/
Abstract

Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.

摘要

细胞内生物制药药物的光触发释放是现代科学中一个具有挑战性的方向，这需要获得新的聚合物体系。制备了聚-L-赖氨酸与肝素的聚电解质复合物（IPECs），其能够包封遗传构建体，如模型寡核苷酸、小干扰RNA（siRNA）和质粒DNA（pDNA）。优化了聚-L-赖氨酸与肝素的比例，以提供从多聚体中释放遗传物质的合适动力学。为了使所得的IPEC具有光敏特性，基于4-溴甲基-3-硝基苯甲酸合成了连接体。仔细研究了光敏连接体破坏的条件和动力学。用Cy3探针修饰IPEC的胶体颗粒，并通过流式细胞术研究其细胞内化。评估了光敏IPECs作为siRNA和pDNA递送系统的功效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/0c3933ad8dc1/polymers-12-01077-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/d31143998cbf/polymers-12-01077-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/ce5b2800296e/polymers-12-01077-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/00eada5f0804/polymers-12-01077-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/cbee313e9b2b/polymers-12-01077-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/9280d974120e/polymers-12-01077-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/58564ef9ec48/polymers-12-01077-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/97fd3a543a86/polymers-12-01077-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/0fe94041beda/polymers-12-01077-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/cce61ff160fc/polymers-12-01077-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/259e30a287e0/polymers-12-01077-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/864c3b5a0fd2/polymers-12-01077-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/0c3933ad8dc1/polymers-12-01077-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/d31143998cbf/polymers-12-01077-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/ce5b2800296e/polymers-12-01077-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/00eada5f0804/polymers-12-01077-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/cbee313e9b2b/polymers-12-01077-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/9280d974120e/polymers-12-01077-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/58564ef9ec48/polymers-12-01077-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/97fd3a543a86/polymers-12-01077-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/0fe94041beda/polymers-12-01077-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/cce61ff160fc/polymers-12-01077-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/259e30a287e0/polymers-12-01077-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/864c3b5a0fd2/polymers-12-01077-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee13/7285230/0c3933ad8dc1/polymers-12-01077-g012.jpg

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本文引用的文献

1
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J Mater Chem B. 2018 Aug 28;6(32):5269-5281. doi: 10.1039/c8tb00994e. Epub 2018 Aug 1.
2
Functionalized Pt(II) and Ir(III) NIR Emitters and Their Covalent Conjugates with Polymer-Based Nanocarriers.功能化的 Pt(II) 和 Ir(III) 近红外发光体及其与基于聚合物的纳米载体的共价轭合物。
Bioconjug Chem. 2020 May 20;31(5):1327-1343. doi: 10.1021/acs.bioconjchem.0c00020. Epub 2020 Apr 9.
3
Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues.
Mil Med Res. 2023 Feb 27;10(1):9. doi: 10.1186/s40779-023-00445-z.
4
Self-Assembling Systems Based on Pillar[5]arenes and Surfactants for Encapsulation of Diagnostic Dye DAPI.基于[5]轮芳烃和表面活性剂的自组装体系用于包裹诊断染料 DAPI。
Int J Mol Sci. 2021 Jun 3;22(11):6038. doi: 10.3390/ijms22116038.
基于脂质体的原位基因递送方法用于将视网膜和大脑皮层作为免疫赦免组织。
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4
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Mol Ther. 2020 Mar 4;28(3):709-722. doi: 10.1016/j.ymthe.2020.01.001. Epub 2020 Jan 10.
5
Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells.用于将VEGF小干扰RNA递送至视网膜上皮细胞的两亲性多肽
Pharmaceutics. 2020 Jan 2;12(1):39. doi: 10.3390/pharmaceutics12010039.
6
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7
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10
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Exp Mol Pathol. 2018 Aug;105(1):1-9. doi: 10.1016/j.yexmp.2018.05.003. Epub 2018 May 15.