Yoneda Arata, Ohtani Misato, Katagiri Daisuke, Hosokawa Yoichiroh, Demura Taku
Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-8562, Japan.
Plants (Basel). 2020 May 9;9(5):604. doi: 10.3390/plants9050604.
Hechtian strands are thread-like structures in plasmolyzed plant cells that connect the cell wall to the plasma membrane. Although these strands were first observed more than 100 years ago, their physiological roles are largely unknown. Here, we used intracellular laser microdissection to examine the effects of disrupting Hechtian strands on plasmolyzed tobacco BY-2 cells. When we focused femtosecond laser pulses on Hechtian strands, targeted disruptions were induced, but no visible changes in cell morphology were detected. However, the calcofluor white signals from β-glucans was detected in plasmolyzed cells with disrupted Hechtian strands, whereas no signals were detected in untreated plasmolyzed cells. These results suggest that Hechtian strands play roles in sensing cell wall integrity.
赫氏丝是质壁分离植物细胞中连接细胞壁和质膜的丝状结构。尽管这些丝在100多年前就首次被观察到，但其生理作用在很大程度上仍不清楚。在这里，我们使用细胞内激光显微切割技术来研究破坏赫氏丝对质壁分离的烟草BY-2细胞的影响。当我们将飞秒激光脉冲聚焦在赫氏丝上时，会诱导靶向破坏，但未检测到细胞形态有明显变化。然而，在赫氏丝被破坏的质壁分离细胞中检测到了来自β-葡聚糖的荧光增白剂信号，而在未处理的质壁分离细胞中未检测到信号。这些结果表明，赫氏丝在感知细胞壁完整性方面发挥作用。
