Porfireva Anna, Evtugyn Gennady
A.M. Butlerov' Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia.
Analytical Chemistry Department of Chemical Technology Institute of Ural Federal University, 19 Mira Street, 620002 Ekaterinburg, Russia.
Nanomaterials (Basel). 2020 May 10;10(5):924. doi: 10.3390/nano10050924.
A DNA sensor has been developed for the determination of doxorubicin by consecutive electropolymerization of an equimolar mixture of Azure B and proflavine and adsorption of native DNA from salmon sperm on a polymer film. Electrochemical investigation showed a difference in the behavior of individual drugs polymerized and their mixture. The use of the copolymer offered some advantages, i.e., a higher roughness of the surface, a wider range of the pH sensitivity of the response, a denser and more robust film, etc. The formation of the polymer film and its redox properties were studied using scanning electron microscopy and electrochemical impedance spectroscopy. For the doxorubicin determination, its solution was mixed with DNA and applied on the polymer surface. After that, charge transfer resistance was assessed in the presence of [Fe(CN)] as the redox probe. Its value regularly grew with the doxorubicin concentration in the range from 0.03 to 10 nM (limit of detection 0.01 nM). The DNA sensor was tested on the doxorubicin preparations and spiked samples mimicking blood serum. The recovery was found to be 98-106%. The DNA sensor developed can find application for the determination of drug residues in blood and for the pharmacokinetics studies.
通过将天青B和硫酸原黄素的等摩尔混合物连续电聚合，并将鲑鱼精子中的天然DNA吸附在聚合物膜上，开发了一种用于测定阿霉素的DNA传感器。电化学研究表明，单独聚合的药物及其混合物的行为存在差异。使用共聚物具有一些优点，即表面粗糙度更高、响应的pH敏感范围更宽、膜更致密且更坚固等。使用扫描电子显微镜和电化学阻抗谱研究了聚合物膜的形成及其氧化还原性质。为了测定阿霉素，将其溶液与DNA混合并施加在聚合物表面。之后，在存在[Fe(CN)]作为氧化还原探针的情况下评估电荷转移电阻。其值在阿霉素浓度为0.03至10 nM范围内有规律地增长（检测限为0.01 nM）。该DNA传感器在阿霉素制剂和模拟血清的加标样品上进行了测试。回收率为98 - 106%。所开发的DNA传感器可用于测定血液中的药物残留以及进行药代动力学研究。
