Halal Research Center of IRI, MOH, Tehran, Iran.
Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
IET Nanobiotechnol. 2022 Jul;16(5):190-198. doi: 10.1049/nbt2.12086. Epub 2022 Apr 20.
A nanocomposite of graphene oxide and gold nanourchins has been used here to modify the surface of a screen-printed carbon electrode to enhance the sensitivity of the electrochemical DNA detection system. A specific single-stranded DNA probe was designed based on the target DNA sequence and was thiolated to be self-assembled on the surface of the gold nanourchins placed on the modified electrode. Doxorubicin was used as an electrochemical label to detect the DNA hybridisation using differential pulse voltammetry (DPV). The assembling process was confirmed using scanning electron microscopy (SEM) imaging, cyclic voltammetry (CV), and the EIS method. The high sensitivity of the proposed system led to a low detection limit of 0.16 fM and a wide linear range from 0.5 to 950.0 fM. The specificity of the DNA hybridisation and the signalling molecule (haematoxylin) caused very high selectivity towards the target DNA than other non-specific sequences.
这里使用氧化石墨烯和金纳米棒的纳米复合材料来修饰丝网印刷碳电极的表面，以提高电化学 DNA 检测系统的灵敏度。根据目标 DNA 序列设计了特定的单链 DNA 探针，并将其巯基化以便自组装在放置在修饰电极上的金纳米棒表面上。阿霉素被用作电化学标记物，通过差分脉冲伏安法（DPV）检测 DNA 杂交。使用扫描电子显微镜（SEM）成像、循环伏安法（CV）和 EIS 方法证实了组装过程。该系统具有高灵敏度，导致检测限低至 0.16 fM，线性范围从 0.5 到 950.0 fM。DNA 杂交和信号分子（苏木精）的特异性导致对目标 DNA 的选择性非常高，高于其他非特异性序列。
