Prokop Jeremy W, Chhetri Surya B, van Veen J Edward, Chen Xuqi, Underwood Adam C, Uhl Katie, Dwinell Melinda R, Geurts Aron M, Correa Stephanie M, Arnold Arthur P
Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, Grand Rapids, MI, 49503, USA.
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, 48824, USA.
Biol Sex Differ. 2020 May 12;11(1):28. doi: 10.1186/s13293-020-00305-8.
The commonly used laboratory rat, Rattus norvegicus, is unique in having multiple Sry gene copies found on the Y chromosome, with different copies encoding amino acid variations that influence the resulting protein function. It is not clear which Sry genes are expressed at the onset of testis differentiation or how their expression correlates with that of other genes in testis-determination pathways.
Here, two independent E11-E14 developmental RNAseq datasets show that multiple Sry genes are expressed at E12-E13.
The identified copies expressed during testis initiation include Sry4A, Sry1, and Sry3C, which are conserved in every strain of Rattus norvegicus with genomes sequenced to date.
This work represents a first step in defining the complex environment of rat testis differentiation that can open the door for generating sex reversal model systems using embryo manipulation techniques that have been available in the mouse but not the rat.
常用实验大鼠褐家鼠的独特之处在于其Y染色体上存在多个Sry基因拷贝，不同拷贝编码的氨基酸变异会影响最终蛋白质的功能。目前尚不清楚哪些Sry基因在睾丸分化开始时表达，以及它们的表达与睾丸决定途径中其他基因的表达如何相关。
在这里，两个独立的E11 - E14发育RNA测序数据集表明，多个Sry基因在E12 - E13表达。
在睾丸起始阶段表达的已鉴定拷贝包括Sry4A、Sry1和Sry3C，在迄今已测序基因组的每一种褐家鼠品系中这些基因都是保守的。
这项工作是定义大鼠睾丸分化复杂环境的第一步，这可为利用小鼠可用但大鼠不可用的胚胎操作技术建立性反转模型系统打开大门。
